The present study reveals the plant growth-promoting (PGP) potentials and characterizes the antifungal metabolites of Kutzneria sp. strain TSII isolated from mangrove sediment soil through in vitro and in silico studies. In this study, Kutzneria sp. strain TSII was screened for PGP activities and the antifungal activities against Pithomyces atro-olivaceous, a leaf spot-associated pathogen in groundnut plants. The ethyl acetate extract of Kutzneria sp. strain TSII was purified using column chromatography, and the presence of various antimicrobial compounds was studied by gas chromatography–mass spectrometry (GC–MS) analysis. In silico modeling and docking were carried out to evaluate the antifungal potent of bioactive compound. Kutzneria sp. strain TSII produced proteases, phosphatases, ammonia, siderophores, cellulases, indole acetic acid (IAA), lipases, and amylases, indicating its ability to enhance the growth of plants. The ethyl acetate extract of Kutzneria sp strain TSII was found to be a potent inhibitor of fungal mycelial growth in the potato dextrose agar (PDA) plates. The GC–MS spectral study showed 24 antimicrobial compounds belonging to five chemical groups: phenolics, phthalates, fatty acid methyl esters (FAME), spiro, and fatty alcohols. In silico docking studies showed that phenol, 2,4-bis(1,1-dimethylethyl)—effectively attaches with the active site of mitochondrial F₁F₀ Adenosine triphosphate synthase enzymes of Pithomyces atro-olivaceous. Hence, it is clear that these antifungal compounds shall be formulated shortly to treat many plant fungal diseases in an eco-friendly manner.

本研究揭示了植物生长促进 (PGP) 的潜力并表征了Kutzneria sp.的抗真菌代谢物。通过体外和计算机研究从红树林沉积物土壤中分离出菌株 TSII。在这项研究中，Kutzneria sp。筛选菌株TSII 的PGP 活性和对花生植物中与叶斑病相关的病原体Pithomyces atro-oliveous的抗真菌活性。Kutzneria sp.的乙酸乙酯提取物。菌株 TSII 使用柱色谱纯化，并通过气相色谱-质谱 (GC-MS) 分析研究各种抗菌化合物的存在。进行了计算机模拟和对接以评估生物活性化合物的抗真菌效力。Kutzneria sp. TSII 菌株产生蛋白酶、磷酸酶、氨、铁载体、纤维素酶、吲哚乙酸 (IAA)、脂肪酶和淀粉酶，表明其具有促进植物生长的能力。发现Kutzneria sp 菌株TSII的乙酸乙酯提取物是马铃薯葡萄糖琼脂 (PDA) 平板中真菌菌丝体生长的有效抑制剂。GC-MS 光谱研究显示 24 种抗菌化合物属于五个化学组：酚类、邻苯二甲酸酯、脂肪酸甲酯 (FAME)、螺和脂肪醇。计算机对接研究表明，苯酚，2,4-双（1,1-二甲基乙基）-有效地与Pithomyces atro-olivously线粒体 F ₁ F ₀三磷酸腺苷合酶的活性位点结合. 因此，很明显，这些抗真菌化合物很快就会被配制出来，以生态友好的方式治疗许多植物真菌病害。