Multifunctional Modification of SIS Membrane with Chimeric Peptides to Promote Its Antibacterial, Osteogenic, and Healing-Promoting Abilities for Applying to GBR,Advanced Functional Materials

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Multifunctional Modification of SIS Membrane with Chimeric Peptides to Promote Its Antibacterial, Osteogenic, and Healing-Promoting Abilities for Applying to GBR
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2021-05-28 , DOI: 10.1002/adfm.202101452
Yuzhu Mu ₁ , Shiqing Ma ₁ , Pengfei Wei ₂ , Yonglan Wang ₁ , Wei Jing ₂ , Yifan Zhao ₁ , Lei Zhang ₁ , Jinzhe Wu ₁ , Bo Zhao ₂ , Jiayin Deng ₁ , Zihao Liu ₁

Affiliation

Guided bone regeneration (GBR) technology is the most widely used and stable method for bone defect repair. However, infectious bone defect limits the application of this technique. Herein, a small intestinal submucosa (SIS) membrane modified by chimeric peptides as a new type of GBR membrane is developed for efficacious tissue regeneration. Based on the main components of SIS membrane are I and III collagen, collagen binding peptides TKKTLRT and KELNLVY sequences are used to construct chimeric peptides with healing-promoting peptide Hst1 or antibacterial osteogenic peptide JH8194, so as to realize the specifically target of SIS. This method achieves the fast and efficient multifunctional modification of SIS membrane. The chimeric peptides modified SIS (pSIS) membrane has satisfactory biocompatibility and a certain degree of antibacterial activity. Moreover, pSIS promotes the osteogenic related factors expression of rat bone mesenchymal stem cells and demonstrates great bone regeneration in rat skull defect model. Furthermore, pSIS accelerates the migration of oral epithelial cells in vitro and activate integrin α3β1 signal pathway contribute to wound healing. This study presents a novel biomaterial design of GBR membrane, specifically for the treatment of infectious bone defects.

中文翻译：

用嵌合肽对 SIS 膜进行多功能修饰以促进其抗菌、成骨和促进愈合的能力，以应用于 GBR

引导骨再生（GBR）技术是目前应用最广泛、最稳定的骨缺损修复方法。然而，传染性骨缺损限制了该技术的应用。在此，开发了一种由嵌合肽修饰的小肠粘膜下层 (SIS) 膜作为一种新型 GBR 膜，用于有效的组织再生。基于SIS膜的主要成分是I和III胶原，利用胶原结合肽TKKTLRT和KELNLVY序列构建与愈合促进肽Hst1或抗菌成骨肽JH8194的嵌合肽，从而实现SIS的特异性靶向。该方法实现了SIS膜的快速高效多功能改性。嵌合肽修饰SIS（pSIS）膜具有令人满意的生物相容性和一定程度的抗菌活性。此外，pSIS 促进大鼠骨间充质干细胞的成骨相关因子表达，并在大鼠颅骨缺损模型中显示出良好的骨再生。此外，pSIS 在体外加速口腔上皮细胞的迁移并激活整合素 α3β1 信号通路有助于伤口愈合。本研究提出了一种新型的 GBR 膜生物材料设计，专门用于治疗感染性骨缺损。

更新日期：2021-08-05

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