Targeted Microfluidic Manufacturing to Mimic Biological Microenvironments: Cell-Encapsulated Hollow Fibers,ACS Macro Letters

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Targeted Microfluidic Manufacturing to Mimic Biological Microenvironments: Cell-Encapsulated Hollow Fibers
ACS Macro Letters ( IF 5.1 ) Pub Date : 2021-05-28 , DOI: 10.1021/acsmacrolett.1c00159
Marilyn C McNamara ₁ , Saurabh S Aykar ₁ , Reza Montazami ₁ , Nicole N Hashemi _{1,

2}

Affiliation

At present, the blood–brain barrier (BBB) poses a challenge for treating a wide range of central nervous system disorders; reliable BBB models are still needed to understand and manipulate the transfer of molecules into the brain, thereby improving the efficiency of treatments. In this study, hollow, cell-laden microfibers are fabricated and investigated as a starting point for generating BBB models. The genetic effects of the manufacturing process are analyzed to understand the implications of encapsulating cells in this manner. These fibers are created using different manufacturing parameters to understand the effects on wall thickness and overall diameter. Then, dopaminergic rat cells are encapsulated into hollow fibers, which maintained at least 60% live cells throughout the three-day observation period. Lastly, genetic changes tyrosine hydroxylase (TH) and tubulin beta 3 class III (TUBB-3) are investigated to elucidate the effects on cell health and behavior; while the TH levels in encapsulated cells were similar to control cells, showing similar levels of TH synthesis, TUBB-3 was downregulated, indicating lower amounts of cellular neurogenesis.

中文翻译：

模拟生物微环境的靶向微流控制造：细胞封装中空纤维

目前，血脑屏障（BBB）对治疗多种中枢神经系统疾病提出了挑战；仍然需要可靠的 BBB 模型来理解和操纵分子向大脑的转移，从而提高治疗效率。在这项研究中，制造并研究了中空的、载有细胞的微纤维，作为生成 BBB 模型的起点。分析制造过程的遗传效应以了解以这种方式封装细胞的含义。这些纤维是使用不同的制造参数创建的，以了解对壁厚和总直径的影响。然后，多巴胺能大鼠细胞被包裹在中空纤维中，在为期三天的观察期内保持至少 60% 的活细胞。最后，研究基因变化酪氨酸羟化酶 (TH) 和微管蛋白 3 类 III (TUBB-3)，以阐明对细胞健康和行为的影响；虽然封装细胞中的 TH 水平与对照细胞相似，显示出相似的 TH 合成水平，但 TUBB-3 被下调，表明细胞神经发生量较低。

更新日期：2021-06-15

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