Hyaloklossia Labbé ,1896 (Alveolata: Apicomplexa) is a monotypic genus of renal coccidia found in anurans, particularly in the edible frog Pelophylax kl. esculentus (Amphibia: Anura: Ranidae), distributed in different parts of Europe. Here we propose a new Hyaloklossia species from the Tokyo daruma pond frog, Pelophylax porosus porosus. The coccidium detected in the renal tissue of P. p. porosus shared some morphological characteristics with the type species, Hyaloklossia lieberkuehni (Labbé, 1894), reported from P. kl. esculentus. However, in addition to size differences in several oocyst and sporocyst features between these parasites, phylogenetic analysis of gene fragments from two nuclear ribosomal loci and the mitochondrial cytochrome c oxidase subunit 1, exposed distinct genetic differences between H. lieberkuehni and our new species. Although our analysis validated the monophyly of Hyaloklossia with some members of the Toxoplasmatinae Biocca, 1957, Cystoisosporinae Frenkel et al., 1987, and Eumonosporinae Chou et al., 2021 (Sarcocystidae Poche, 1913), comparison of genetic differences between Hyaloklossia species from P. p. porosus and H. lieberkuehni revealed the presence of a greater number of polymorphisms than that observed when comparing inter-species (Heydornia spp., Besnoisita spp.) or inter-genus (Toxoplasma vs. Neospora, Neospora vs. Hammondia, and Neospora vs. Heydornia) variabilities among members of the Sarcocystidae. This indicates that Hyaloklossia, as re-erected and defined by Modrý et al. (2001, Int. J. Syst. Evol. Microbiol. 51, 767–772), with its homoxenous life cycle, requires placement in its own subfamily. Thus, we propose a new subfamily, Hyaloklossiinae n. subfam., to accommodate two species, H. lieberkuehni from Europe and Hyaloklossia kasumienesis n. sp. which we describe here from P. p. porosus in Japan.

Hyaloklossia  Labbé ,1896 (Alveolata: Apicomplexa) 是一种肾球虫的单型属，在无尾目中发现，特别是在可食用的青蛙Pelophylax kl 中。esculentus (Amphibia: Anura: Ranidae)，分布于欧洲不同地区。在这里，我们提出了一种来自东京达摩池蛙Pelophylax porosus porosus的新Hyaloklossia物种。在P. p.的肾组织中检测到球虫。porosus与模式种Hyaloklossia lieberkuehni (Labbé, 1894)共享一些形态特征，来自P. kl。香菜. 然而，除了这些寄生虫之间几个卵囊和孢子囊特征的大小差异外，来自两个核核糖体基因座和线粒体细胞色素c氧化酶亚基 1的基因片段的系统发育分析揭示了 H.lieberkuehni和我们的新物种之间的明显遗传差异。虽然我们的分析验证的单系Hyaloklossia与Toxoplasmatinae Biocca，1957年的一些成员，Cystoisosporinae Frenkel等人，1987，和Eumonosporinae Chou等，2021（Sarcocystidae Poche，1913）之间的遗传差异比较Hyaloklossia从物种P .p. porosus和H.lieberkuehni揭示多态性比比较种间时所观察到的更大数量的存在（Heydornia属，Besnoisita属）或帧间属（弓形虫VS 。新孢子虫，新孢子虫对Hammondia，和新孢子虫VS 。Heydornia）变率成员之间肉蚧科的。这表明Hyaloklossia，作为重新竖立并通过Modrý等人定义。(2001, Int. J. Syst. Evol. Microbiol. 51, 767–772) 具有同种生命周期，需要置于其自己的亚科中。因此，我们提出了一个新的亚科 Hyaloklossiinae n。subfam.，容纳两个物种，来自欧洲的H. lieberkuehni和Hyaloklossia kasumienesis n. sp. 我们在这里从P. p. 多孔在日本。