As one kind of important disinfectant and reactive oxygen species (ROS), hypochlorite (ClO^-), plays vital roles in both water treatment and cell homeostasis. In this work, by decorating a series of groups with different electron donating and withdrawing properties on tetraphenylethene (TPE), four aggregation-induced emission (AIE)-based fluorescent probes containing C˭C double bonds as the potential reaction sites named Probe A, B, C and D were constructed, and their sensing performance for ClO^- was systematically studied. The results showed that the substituents can not only effectively tune the photophysical properties of the probes, but also make a significant impact on their sensing performance for ClO^-. Combined with the theoretical calculation results, it can be inferred that the reactivity of the probes for ClO^- can be greatly enhanced with the increase of electron cloud density on the C˭C double bonds by the introduction of strong electron-donating group (EDG) and electron-withdrawing group (EWG) adjacent to the double bonds. Finally, the best performing Probe D was selected and then successfully applied to ClO^- detection in real water samples and live cell imaging.

作为一种重要的消毒剂和活性氧 (ROS)，次氯酸盐 (ClO ^- ) 在水处理和细胞稳态中起着至关重要的作用。在这项工作中，通过在四苯乙烯（TPE）上装饰一系列具有不同给电子和吸电子特性的基团，四种含有C˭C双键作为潜在反应位点的基于聚集诱导发射（AIE）的荧光探针命名为探针A，构建了 B、C 和 D，并系统地研究了它们对 ClO ^-的传感性能。结果表明，取代基不仅可以有效地调整探头的光物理性质，也使对他们的CLO感性能有显著的影响^-. 与理论计算结果相结合，它可以推断出探针为CLO的反应性^-可大大与电子云密度对C = C双键通过引入强给电子基团的增加而增强（EDG）和与双键相邻的吸电子基团（EWG）。最后，为表现最佳探头d被选择，然后成功地应用于至C10 ^-检测实际水样并活细胞成像英寸