用于检测活细胞中 HClO 的新型线粒体靶向和基于萘酰亚胺的荧光探针,ACS Omega

当前位置： X-MOL 学术 › ACS Omega › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

用于检测活细胞中 HClO 的新型线粒体靶向和基于萘酰亚胺的荧光探针
ACS Omega ( IF 3.7 ) Pub Date : 2021-05-26 , DOI: 10.1021/acsomega.1c01271
Junhong Xu ₁ , Chunyan Wang ₂ , Qiujuan Ma ₂ , Hongtao Zhang ₁ , Meiju Tian ₂ , Jingguo Sun ₂ , Baiyan Wang ₃ , Yacong Chen ₂

Affiliation

作为一种关键的活性氧 (ROS)，次氯酸 (HClO) 在许多生理和病理过程中起着重要作用。用于高灵敏度检测 HClO 的线粒体靶向探针是可取的。在目前的工作中，我们设计并合成了一种用于检测 HClO 的原始线粒体定位和开启荧光探针。4-氨基萘二甲酰亚胺用作荧光部分，(2-氨基乙基)-硫脲单元用作典型的传感单元，季铵化吡啶部分用作线粒体靶向定位基团。当 HClO 不存在时，探针显示弱荧光。在 HClO 存在下，探针显示蓝色荧光。此外，开启的荧光探针能够在广泛的 pH 范围内发挥作用。^–7至 2.5 × 10 ^–6 mol·L ^–1。此外，该探针几乎没有细胞毒性，并具有出色的线粒体定位能力。此外，该探针能够对活 PC-12 细胞线粒体中的 HClO 成像。上述显着特性表明该探针能够测定活细胞线粒体中的 HClO。

"点击查看英文标题和摘要"

Novel Mitochondria-Targeting and Naphthalimide-based Fluorescent Probe for Detecting HClO in Living Cells

As a key reactive oxygen species (ROS), hypochlorous acid (HClO) plays an important role in many physiological and pathological processes. The mitochondria-targeting probes for the highly sensitive detection of HClO are desirable. In present work, we designed and synthesized an original mitochondria-localizing and turn-on fluorescent probe for detecting HClO. 4-Aminonaphthalimide was employed as the fluorescent section, the (2-aminoethyl)-thiourea unit was utilized as a typical sensing unit, and the quaternized pyridinium moiety was used as a mitochondria-targeted localization group. When HClO was absent, the probe showed weak fluorescence. In the existence of HClO, the probe revealed a blue fluorescence. Moreover, the turn-on fluorescent probe was able to function in a broad pH scope. There was an excellent linearity between the fluorescence emission intensity at 488 nm and the concentrations of HClO in the range of 5.0 × 10^–7 to 2.5 × 10^–6 mol·L^–1. Additionally, the probe had almost no cell toxicity and possessed an excellent mitochondria-localizing capability. Furthermore, the probe was able to image HClO in mitochondria of living PC-12 cells. The above remarkable properties illustrated that the probe was able to determine HClO in mitochondria of living cells.

更新日期：2021-06-08

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南