As an important industrial chemical and promising biofuel, biobutanol has attracted extensive attention while still facing the challenges of low production efficiency and yield. The major obstacle comes from product inhibition by high concentration of butanol, therefore in this study pervaporation was used and integrated with acetone-butanol-ethanol fermentation by Clostridium beijerinckii ZL01 to achieve in situ butanol removal from fermentation broth and relieve the inhibition by butanol accumulation. By optimization of integration time, flow rate and initial glucose concentration, the optimal conditions were determined as integration time of 12 h, flow rate of 30 L/h and initial glucose concentration of 90 g/L. The integration of pervaporation dramatically shortened the overall fermentation time from 70 h to 40 h, and increased the total solvents production from 14.30 g/L to 30.83 g/L and the sugar-to-solvent conversion ratio of C. beijerinckii ZL01 from 0.16 g/g to 0.34 g/g for batch fermentation. The adoption of fed-batch fermentation further improved the total solvents concentration to 61.32 g/L and sugar-to-solvent conversion ratio to 0.44 g/g. An unusual accumulation of ethanol was observed in the late stage of fed-batch fermentation, which requires further investigation.

作为重要的工业化学品和有前途的生物燃料，生物丁醇已引起广泛关注，同时仍面临生产效率和产量低的挑战。主要障碍来自高浓度丁醇对产物的抑制作用，因此在本研究中，使用了全蒸发，并与拜氏梭状芽胞杆菌ZL01的丙酮-丁醇-乙醇发酵工艺集成，以实现原位生产。从发酵液中除去丁醇并缓解丁醇积累的抑制作用。通过优化积分时间，流速和初始葡萄糖浓度，确定最佳条件为积分时间为12 h，流速为30 L / h和初始葡萄糖浓度为90 g / L。渗透蒸发的整合将整个发酵时间从70 h大大缩短到40 h，并将总溶剂产量从14.30 g / L增加到30.83 g / L，以及拜氏梭菌的糖-溶剂转化率ZL01为0.16 g / g至0.34 g / g，用于分批发酵。分批补料发酵的采用进一步提高了总溶剂浓度至61.32 g / L，糖与溶剂的转化率达到0.44 g / g。分批补料发酵后期观察到乙醇的异常积累，需要进一步研究。