Abstract

Two new hasubanan alkaloids, stephalonester A (1) and stephalonester B (2), together with four known compounds, stephalonine E (3), longanone (4), cephatonine (5), and prostephabyssine (6) were isolated from the whole plant of Stephania longa. Their structures were determined by HR-ESI-MS, 1 D and 2 D NMR, ECD calculations, as well as by comparison with literature values. All compounds were evaluated for their anti-inflammatory activity in vitro. Compounds 4, 5, and 6 exhibited significantly inhibitory effects on TNF-α and IL-6 production with IC₅₀ values range from 6.54 to 30.44 µM.

摘要

从全植物中分离出两种新的哈苏巴南生物碱，stephalonester A ( 1 ) 和 stephalonester B ( 2 )，以及四种已知化合物，stephalonine E ( 3 )、longanone ( 4 )、cephatonine ( 5 ) 和 prostephabyssine ( 6 )的Stephania longa。它们的结构通过 HR-ESI-MS、1D 和 2D NMR、ECD 计算以及与文献值的比较来确定。在体外评估了所有化合物的抗炎活性。化合物4、5和6对 TNF-α 和 IL- 6的产生具有显着的抑制作用，IC ₅₀值范围从 6.54 到 30.44 µM。