During the past decades, extracellular vesicles (EVs) have emerged as an attractive drug delivery system. Here, we assess their pre-clinical applications, in the form of a systematic review. For each study published in the past decade, disease models, animal species, EV donor cell types, active pharmaceutical ingredients (APIs), EV surface modifications, API loading methods, EV size and charge, estimation of EV purity, presence of biodistribution studies and administration routes were quantitatively analyzed in a defined and reproducible way. We have interpreted the trends we observe over the past decade, to define the niches where to apply EVs for drug delivery in the future and to provide a basis for regulatory guidelines.

在过去的几十年中，细胞外囊泡 (EV) 已成为一种有吸引力的药物输送系统。在这里，我们以系统评价的形式评估它们的临床前应用。对于过去十年发表的每项研究，疾病模型、动物物种、EV 供体细胞类型、活性药物成分 (API)、EV 表面改性、API 加载方法、EV 大小和电荷、EV 纯度估计、生物分布研究的存在和以明确且可重复的方式对给药途径进行定量分析。我们解释了我们在过去十年中观察到的趋势，以确定未来在哪些领域应用电动汽车进行药物输送，并为监管指南提供基础。