Recent advances in activity-based probes (ABPs) and affinity-based probes (AfBPs) for profiling of enzymes,Chemical Science

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Recent advances in activity-based probes (ABPs) and affinity-based probes (AfBPs) for profiling of enzymes
Chemical Science ( IF 7.6 ) Pub Date : 2021-5-18 , DOI: 10.1039/d1sc01359a
Haixiao Fang ₁ , Bo Peng ₂ , Sing Yee Ong ₃ , Qiong Wu ₁ , Lin Li ₁ , Shao Q Yao ₃

Affiliation

Activity-based protein profiling (ABPP) is a technique that uses highly selective active-site targeted chemical probes to label and monitor the state of proteins. ABPP integrates the strengths of both chemical and biological disciplines. By utilizing chemically synthesized or modified bioactive molecules, ABPP is able to reveal complex physiological and pathological enzyme–substrate interactions at molecular and cellular levels. It is also able to provide critical information of the catalytic activity changes of enzymes, annotate new functions of enzymes, discover new substrates of enzymes, and allow real-time monitoring of the cellular location of enzymes. Based on the mechanism of probe-enzyme interaction, two types of probes that have been used in ABPP are activity-based probes (ABPs) and affinity-based probes (AfBPs). This review highlights the recent advances in the use of ABPs and AfBPs, and summarizes their design strategies (based on inhibitors and substrates) and detection approaches.

中文翻译：

用于酶分析的基于活性的探针 (ABP) 和基于亲和力的探针 (AfBP) 的最新进展

基于活性的蛋白质分析 (ABPP) 是一种使用高选择性活性位点靶向化学探针来标记和监测蛋白质状态的技术。ABPP 综合了化学和生物学科的优势。通过利用化学合成或修饰的生物活性分子，ABPP 能够在分子和细胞水平上揭示复杂的生理和病理酶-底物相互作用。它还能够提供酶催化活性变化的关键信息，注释酶的新功能，发现酶的新底物，并允许实时监测酶的细胞位置。基于探针-酶相互作用的机制，ABPP中使用的探针有两种类型：基于活性的探针（ABP）和基于亲和力的探针（A f BP）。本综述重点介绍了 ABP 和 A f BP使用的最新进展，并总结了它们的设计策略（基于抑制剂和底物）和检测方法。

更新日期：2021-05-18

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