In the study, biotin-modified oligochitosan-F127 conjugate (F127-CS-BIO) was synthesized as a carrier to prepare honokiol-loaded micelles via dialysis method. The encapsulation efficiency and drug loading were 70.79 ± 2.70% and 9.70 ± 0.57%, respectively. The mean particle size and zeta potentials were 210.9 ± 4.65 nm and 12.9 ± 7.22 mV, respectively. In vitro release studies showed that the release of honokiol from the micelles was slow and steady. MTT evaluation and cellular uptake results demonstrated that F127-CS-BIO was more easily absorbed by tumor cells and resulted in low IC₅₀ value of honokiol due to biotin’s targeting guidance when compared with biotin-unmodified F127-CS. In conclusion, F127-CS-BIO polymer was expected as an effective nanocarrier to encapsulate chemotherapeutic drugs.

Graphical abstract

中文翻译：

---

生物素修饰的寡聚壳聚糖-F127胶束用于厚朴酚的包封

在这项研究中，合成生物素修饰的寡聚壳聚糖-F127共轭物（F127-CS-BIO）作为载体，通过透析方法制备负载厚朴酚的胶束。包封效率和载药量分别为70.79±2.70％和9.70±0.57％。平均粒径和Zeta电位分别为210.9±4.65 nm和12.9±7.22 mV。体外释放研究表明，厚朴酚从胶束中的释放缓慢而稳定。MTT评估和细胞摄取结果表明，与未经生物素修饰的F127-CS相比，由于生物素的靶向指导，F127-CS-BIO更容易被肿瘤细胞吸收，并且导致厚朴酚的IC ₅₀值低。总之，F127-CS-BIO聚合物有望作为封装化疗药物的有效纳米载体。

图形概要