酰胺 I 谱带在偏振拉曼光谱、红外光谱和振动二色光谱中的组合用于肽原纤维和无序肽和蛋白质的结构分析,Journal of Raman Spectroscopy

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酰胺 I 谱带在偏振拉曼光谱、红外光谱和振动二色光谱中的组合用于肽原纤维和无序肽和蛋白质的结构分析
Journal of Raman Spectroscopy ( IF 2.4 ) Pub Date : 2021-05-15 , DOI: 10.1002/jrs.6137
Reinhard Schweitzer‐Stenner ₁

Affiliation

拉曼光谱通常是探索溶液中蛋白质和肽结构的通用工具。然而，尽管开发了紫外 (UV) 共振拉曼光谱作为研究低浓度样品的工具，但拉曼在二级结构分析方面并没有发挥与红外 (IR) 光谱相同的作用。报道的 UV 拉曼研究最近集中在酰胺 III 的 ψ 依赖性上，作为一种有用的工具，即使是无序肽的结构分析。基于酰胺 I 的结构分析通常使用可见激发。而不是描述蛋白质的传统二级结构分析，本综述侧重于使用拉曼光谱与其他振动光谱学（如 IR 和振动圆二色性）结合来探测未折叠肽中氨基酸残基的构象分布以及肽自组装成原纤维的工作。我们反对使用光谱分解成高斯波段，特别是如果这种类型的分析专门应用于 IR 或拉曼波段轮廓。这篇文章强调了酰胺 I 波函数的离域激子特性，在使用各自的波段进行结构分析的任何尝试中都必须考虑到这一点。我们反对使用光谱分解成高斯波段，特别是如果这种类型的分析专门应用于 IR 或拉曼波段轮廓。这篇文章强调了酰胺 I 波函数的离域激子特性，在使用各自的波段进行结构分析的任何尝试中都必须考虑到这一点。我们反对使用光谱分解成高斯波段，特别是如果这种类型的分析专门应用于 IR 或拉曼波段轮廓。这篇文章强调了酰胺 I 波函数的离域激子特性，在使用各自的波段进行结构分析的任何尝试中都必须考虑到这一点。

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The combined use of amide I bands in polarized Raman, IR, and vibrational dichroism spectra for the structure analysis of peptide fibrils and disordered peptides and proteins

Raman spectroscopy is generally a versatile tool to explore the structure of proteins and peptides in solution. However, in spite of the development of ultraviolet (UV) resonance Raman spectroscopy as a tool that allows for the investigation of samples with lower concentrations, Raman has not played the same role as infrared (IR) spectroscopy with regard to secondary structure analysis. Reported UV Raman studies have recently focused on the ψ-dependence of amide III as a useful tool for the structural analysis even of disordered peptides. Amide I based structural analysis generally uses visible excitation. Rather than describing the traditional secondary structure analysis of proteins, this review focusses on work that used Raman in conjunction with other vibrational spectroscopies such as IR and vibrational circular dichroism to probe the conformational distribution of amino acid residues in unfolded peptides and the self-assembling of peptides into fibrils. We argue against the use of spectral decomposition into Gaussian bands particularly if this type of analysis is exclusively applied to either IR or Raman band profiles. The article emphasizes the delocalized excitonic character of amide I wavefunctions which has to be accounted for in any attempt to use the respective bands for structure analysis.

更新日期：2021-05-15

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