Multiplex Electrochemiluminescence Polarization Assay Based on the Surface Plasmon Coupling Effect of Au NPs and Ag@Au NPs,Analytical Chemistry

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Multiplex Electrochemiluminescence Polarization Assay Based on the Surface Plasmon Coupling Effect of Au NPs and Ag@Au NPs
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-05-13 , DOI: 10.1021/acs.analchem.1c00757
Zihui Liang ₁ , Yixin Nie ₁ , Xin Zhang ₁ , Peilin Wang ₁ , Qiang Ma ₁

Affiliation

A novel multiplex electrochemiluminescence (ECL) polarization assay was developed to detect breast cancer-related genes BRCA1 and BRCA2 simultaneously based on the polarization characteristics of surface plasmon-coupled electrochemiluminescence (SPC-ECL). In this work, boron nitride quantum dots (BN QDs) were used as ECL emitters, and gold nanoparticles (Au NPs) and gold-coated silver nanoparticles (Ag@Au NPs) were employed as surface plasmon materials. The surface plasmon coupling resonance of different metal NPs not only enhanced the ECL intensity but also converted the isotropic emission into directional emission. This study revealed the relation between the structure of metal nanomaterials and SPC-ECL, and a high polarization-resolved sensing system was designed to detect multitarget DNA from 100 aM to 1 nM simultaneously. Polarization-based multiple ECL analysis has broad prospects in related cancer diagnosis and treatment evaluation.

中文翻译：

基于金纳米粒子和银@金纳米粒子的表面等离子体耦合效应的多重电化学发光极化测定

基于表面等离激元耦合电化学发光（SPC-ECL）的极化特性，开发了一种新颖的多重电化学发光（ECL）极化测定法，以同时检测与乳腺癌相关的基因BRCA1和BRCA2。在这项工作中，氮化硼量子点（BN QDs）被用作ECL发射极，金纳米颗粒（Au NPs）和镀金的银纳米颗粒（Ag @ Au NPs）被用作表面等离子体材料。不同金属纳米粒子的表面等离子体激元耦合共振不仅增强了ECL强度，而且将各向同性发射转变为定向发射。这项研究揭示了金属纳米材料的结构与SPC-ECL之间的关系，并设计了一种高偏振分辨传感系统，可同时检测100 aM至1 nM的多靶DNA。

更新日期：2021-05-25

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