Plant Cell Reports ( IF 5.3 ) Pub Date : 2021-05-13 , DOI: 10.1007/s00299-021-02702-8 Fatma Masmoudi 1 , Slim Tounsi 1 , Christopher A Dunlap 2 , Mohamed Trigui 3
|
Key message
Bacillus spizizenii is for the first time described as a plant growth salt-tolerant bacterium able to alleviate salt stress in crop plants by improving physiological parameters and antioxidant defense mechanisms.
Abstract
Agricultural soil salinization is a serious issue worldwide affecting agricultural yield. Plant growth promoting bacteria can enhance salt tolerance and plant yield. Bacillus spizizenii FMH45 has been shown to inhibit fungal attacks in tomato fruits and to augment tomato seed germination in presence of abiotic stresses. During this study, we reported for the first time B. spizizenii as a salt-tolerant bacterium able to alleviate salt stress in tomato plants. B. spizizenii FMH45 was examined in vitro for its potential to produce several plant growth promoting characters (siderophores, IAA, and phosphate solubilization) and hydrolytic enzymes (cellulase, glucanase and protease) in the presence of saline conditions. FMH45 was also investigated in vivo in pot experiments to evaluate its ability to promote tomato plant growth under salt stress condition. FMH45 inoculation, enhanced tomato seedling length, vigor index, and plant fresh and dry weights when compared to the non-inoculated controls exposed and not exposed to a regular irrigation with salt solutions containing: 0; 3.5; 7; and 10 g L−1 of NaCl. FMH45-treated plants also presented improved chlorophyll content, membrane integrity (MI), and phenol peroxidase (POX) concentrations, as well as reduced malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels under saline conditions with a significant salinity × strain interaction. Furthermore, FMH45 inoculation significantly decreased endogenous Na+ accumulation, increased K+ and Ca2+ uptake, and thereby improved K+/Na+ and Ca2+/Na+ ratios. This study proves that bio-inoculation of FMH45 efficiently increases salt tolerance in tomato plants. This sustainable approach can be applied to other stressed plant species in affected soils.
Graphic abstract
中文翻译：
耐盐螺旋芽孢杆菌 FMH45 促进盐胁迫下番茄植物的生长、生理和抗氧化参数
关键信息
Bacillus spizizenii首次被描述为一种植物生长的耐盐细菌，能够通过改善生理参数和抗氧化防御机制来缓解作物植物的盐胁迫。
抽象的
农业土壤盐渍化是世界范围内影响农业产量的严重问题。植物生长促进细菌可以提高耐盐性和植物产量。Bacillus spizizenii FMH45 已被证明可抑制番茄果实中的真菌侵袭，并在存在非生物胁迫的情况下增强番茄种子的萌发。在这项研究中，我们首次报道了B. spizizenii作为一种能够缓解番茄植物盐胁迫的耐盐细菌。B. spizizeni在体外检测了 FMH45 在盐水条件下产生多种植物生长促进特性（铁载体、IAA 和磷酸盐溶解）和水解酶（纤维素酶、葡聚糖酶和蛋白酶）的潜力。还在盆栽实验中对 FMH45 进行了体内研究，以评估其在盐胁迫条件下促进番茄植物生长的能力。与暴露和未暴露于盐溶液定期灌溉的未接种对照相比，FMH45 接种、增强的番茄幼苗长度、活力指数和植物鲜重和干重： 0；3.5；7; 和 10 g L -1氯化钠。FMH45 处理的植物还表现出改善的叶绿素含量、膜完整性 (MI) 和苯酚过氧化物酶 (POX) 浓度，以及在盐度显着的盐分条件下降低丙二醛 (MDA) 和过氧化氢 (H 2 O 2 ) 水平 ×应变相互作用。此外，FMH45 接种显着降低了内源性 Na +的积累，增加了 K +和 Ca 2+的吸收，从而改善了 K + /Na +和 Ca 2+ /Na +比率。本研究证明，生物接种 FMH45 可有效提高番茄植株的耐盐性。这种可持续的方法可以应用于受影响土壤中的其他受胁迫植物物种。
京公网安备 11010802027423号