Detection of lipase activity in human serum based on a ratiometric fluorescent probe,New Journal of Chemistry

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Detection of lipase activity in human serum based on a ratiometric fluorescent probe
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2021-4-22 , DOI: 10.1039/d1nj01155c
Jiajie Luo _{1,

2,

3,

4} , Hongyi Zhang _{1,

2,

3,

4} , Jialiang Guan _{4,

5,

6,

7} , Baoshuai An _{1,

2,

3,

4} , Junli Peng _{1,

2,

3,

4} , Wei Zhu _{1,

2,

3,

4} , Ningning Wei _{1,

2,

3,

4} , Yanru Zhang _{1,

2,

3,

4}

Affiliation

Acute pancreatitis results in major mortality and morbidity. Lipase is a vital biomarker of acute pancreatitis in the early stage. Developing a simple and convenient methodology for monitoring the lipase activity is of significance to the early diagnosis of acute pancreatitis. In this work, we designed a novel ratiometric fluorescent probe (CARA) based on FRET for detecting lipase activity in human serum. The energy donor group coumarin derivative (CA) and the energy acceptor group rhodamine derivative (RA) were linked for the construction of a coumarin–rhodamine platform through an ester bond that is easily cleaved by lipase, breaking the FRET process between CA and RA. CARA shows a high selectivity to lipase over other biological substances and a good linearity between the fluorescence ratio and lipase activity in serum samples. CARA has the potential to be applied in the clinical diagnosis of acute pancreatitis.

中文翻译：

基于比例荧光探针的人血清中脂肪酶活性检测

急性胰腺炎会导致严重的死亡率和发病率。脂肪酶是早期急性胰腺炎的重要生物标志物。开发一种简单而方便的方法来监测脂肪酶的活性对急性胰腺炎的早期诊断具有重要意义。在这项工作中，我们设计了一种基于FRET的新型比例荧光探针（CARA），用于检测人血清中的脂肪酶活性。能量供体基团香豆素衍生物（CA）和能量受体基团罗丹明衍生物（RA）通过易于被脂肪酶裂解的酯键连接在一起，从而构建了香豆素-若丹明平台，从而打破了CA和RA之间的FRET过程。与其他生物物质相比，CARA对脂肪酶具有很高的选择性，并且血清样品中的荧光比和脂肪酶活性之间具有良好的线性关系。

更新日期：2021-05-13

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