白杨素通过调节胰岛素抵抗 HepG2 细胞和 HFD/STZ 诱导的 C57BL/6J 小鼠中的 AMPK/PI3K/AKT 信号通路改善葡萄糖和脂质代谢紊乱,Journal of Agricultural and Food Chemistry

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白杨素通过调节胰岛素抵抗 HepG2 细胞和 HFD/STZ 诱导的 C57BL/6J 小鼠中的 AMPK/PI3K/AKT 信号通路改善葡萄糖和脂质代谢紊乱
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2021-05-12 , DOI: 10.1021/acs.jafc.1c01109
Ying-Jun Zhou ₁ , Nuo Xu ₁ , Xiao-Chen Zhang ₁ , Yu-Yan Zhu ₁ , Shao-Wei Liu ₁ , Ya-Ning Chang ₁

Affiliation

据报道，副作用较小的天然产物是治疗糖脂代谢紊乱的有效辅助疗法。白杨素是一种黄酮类化合物，具有广泛的生理作用，如抗氧化、抗炎、抗糖尿病、抗高血脂和保肝。本研究旨在利用胰岛素抵抗HepG2细胞和HFD/STZ诱导的C57BL/6J小鼠探讨白杨素对代谢综合征的影响和机制。结果表明，白杨素显着降低了胰岛素抵抗、氧化应激、炎症和肝损伤。此外，白杨素通过调节 GSK3β、G6Paes、PEPCK、SREBP1、FAS 和 ACC1 改善糖原合成和脂肪酸氧化，抑制糖异生和脂肪酸合成。此外，蛋白质印迹和实时荧光定量 PCR 实验结果表明，白杨素通过 AMPK/PI3K/AKT 信号通路调节葡萄糖和脂质代谢。用AMPK抑制剂处理证实AMPK活化与白杨素对糖脂代谢的活性呈正相关。这项研究证实，白杨素是一种潜在的治疗糖脂代谢紊乱的药物。

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Chrysin Improves Glucose and Lipid Metabolism Disorders by Regulating the AMPK/PI3K/AKT Signaling Pathway in Insulin-Resistant HepG2 Cells and HFD/STZ-Induced C57BL/6J Mice

Natural products with minor side effects have been reported to be an effective adjuvant therapy for glucose and lipid metabolism disorders. Chrysin, a flavone, has a wide range of physiological effects, such as antioxidant, anti-inflammatory, anti-diabetes, anti-hyperlipidemia, and hepatoprotective. This study was designed to explore the effects and mechanism of chrysin on metabolic syndrome using insulin-resistant HepG2 cells and HFD/STZ-induced C57BL/6J mice. The results indicated that chrysin significantly decreased insulin resistance, oxidative stress, inflammation, and liver injury. In addition, chrysin improved glycogen synthesis and fatty acid oxidation and inhibited gluconeogenesis and fatty acid synthesis by regulating GSK3β, G6Paes, PEPCK, SREBP1, FAS, and ACC1. Furthermore, the results of western blot and real-time PCR experiments demonstrated that chrysin modulated glucose and lipid metabolism through the AMPK/PI3K/AKT signaling pathway. Treatment with the AMPK inhibitor verified that AMPK activation is positively correlated with chrysin activity on glycolipid metabolism. This study confirms that chrysin is a potential treatment for glucose and lipid metabolism disorders.

更新日期：2021-05-26

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