An aggregation-induced emission (AIE) fluorescent protein chromophore-based probe (CBZ-FP) for detection of human carboxylesterases (CESs) was designed and synthesized. CBZ-FP exhibited good cell permeability with a large stokes shift (116 nm) and can be applied to reveal the actual activities of CES1 in living cells associated with pesticides detoxification process. CBZ-FP can also serve as a fluorescence indicator of pesticide exposure in the way of hydrolyzing the carboxylic acid ester group in CBZ-FP. Therefore, CBZ-FP has high selectivity for CESs and can detect real-time activity of CES1 in biological samples. Molecular docking study was used to explore the binding of CESs and CBZ-FP. Finding that only one specific activity site of CESs can bind with probe. In view of the fact that, the biotransformation of drugs and poisons containing ester groups can carry out normally depending on CESs, Carboxylesterase probes are expected to contribute to the characterization of relevant disease.

设计并合成了一种用于检测人羧酸酯酶（CES）的基于聚集诱导发射（AIE）荧光蛋白发色团的探针（CBZ-FP）。CBZ-FP表现出良好的细胞渗透性，具有较大的斯托克斯位移（116 nm），可用于揭示CES1在与农药解毒过程相关的活细胞中的实际活性。CBZ-FP还可以通过水解CBZ-FP中的羧酸酯基团的方式用作农药暴露的荧光指示剂。因此，CBZ-FP对CES具有很高的选择性，并且可以检测生物样品中CES1的实时活性。分子对接研究用于探索CES与CBZ-FP的结合。发现只有一个特定的CES活动位点可以与探针结合。鉴于这一事实，