Lignin, which is a component of wood, is difficult to degrade in nature. However, serious decay caused by microbial consortia can happen to wooden antiques during the preservation process. This study successfully screened four microbial consortia with lignin degradation capabilities (J-1, J-6, J-8 and J-15) from decayed wooden antiques. Their compositions were identified by genomic sequencing, while the degradation products were analyzed by GC-MS. The lignin degradation efficiency of J-6 reached 54% after 48 h with an initial lignin concentration of 0.5 g/L at pH 4 and rotation speed of 200 rpm. The fungal consortium of J-6 contained Saccharomycetales (98.92%) and Ascomycota (0.56%), which accounted for 31% of the total biomass. The main bacteria in J-6 were Shinella sp. (47.38%), Cupriavidus sp. (29.84%), and Bosea sp. (7.96%). The strongest degradation performance of J-6 corresponded to its composition, where Saccharomycetales likely adapted to the system and improved lignin degradation enzymes activities, and the abundant bacterial consortium accelerated lignin decomposition. Our work demonstrated the potential utilization of microbial consortia via the synergy of microbial consortia, which may overcome the shortcomings of traditional lignin biodegradation when using a single strain, and the potential use of J-6 for lignin degradation/removal applications.

中文翻译：

---

木质古董中木质素降解菌群的筛选与比较

木质素是木材的组成部分，很难在自然界降解。然而，在保存过程中，木质古董可能会发生由微生物菌群引起的严重腐烂。这项研究成功地从腐烂的木制古董中筛选了四个具有木质素降解能力的微生物联盟（J-1，J-6，J-8和J-15）。通过基因组测序鉴定其组成，同时通过GC-MS分析降解产物。J-6的木质素降解效率在48 h后达到54％，初始pH为4时木质素浓度为0.5 g / L，转速为200 rpm。J-6的真菌财团包含酵母菌（98.92％）和子囊菌（0.56％），占生物量的31％。J-6中的主要细菌是Shinella sp.。（47.38％），Cupriavidussp。（29.84％），和氧化包西氏菌。（7.96％）。J-6最强的降解性能与其组成相对应，其中糖酵母可能适应了该系统并改善了木质素降解酶的活性，而丰富的细菌财团则促进了木质素的分解。我们的工作证明了通过微生物联合体的协同作用对微生物联合体的潜在利用，这可以克服使用单一菌株时传统木质素生物降解的缺点，以及J-6在木质素降解/去除应用中的潜在用途。