Ultra-high-performance liquid chromatography high-resolution mass spectrometry (UHPLC–HRMS) variants currently represent the best tools to tackle the challenges of complexity and lack of comprehensive coverage of the metabolome. UHPLC offers flexible and efficient separation coupled with high-sensitivity detection via HRMS, allowing for the detection and identification of a broad range of metabolites. Here we discuss current common strategies for UHPLC–HRMS-based metabolomics, with a focus on expanding metabolome coverage.

中文翻译：

---

用于代谢组学研究的超高效液相色谱高分辨率质谱变体

超高效液相色谱高分辨率质谱 (UHPLC-HRMS) 变体目前代表了应对代谢组复杂性和缺乏全面覆盖的挑战的最佳工具。UHPLC 提供灵活、高效的分离以及通过 HRMS 的高灵敏度检测，允许检测和鉴定范围广泛的代谢物。在这里，我们讨论当前基于 UHPLC-HRMS 的代谢组学的常用策略，重点是扩大代谢组覆盖范围。