<br>安石榴苷通过介导 FOXO3 信号轴减轻 TNF-α 和 IL-1β 诱导的软骨细胞功能障碍和软骨代谢,Journal of Food Biochemistry

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安石榴苷通过介导 FOXO3 信号轴减轻 TNF-α 和 IL-1β 诱导的软骨细胞功能障碍和软骨代谢
Journal of Food Biochemistry ( IF 3.5 ) Pub Date : 2021-05-11 , DOI: 10.1111/jfbc.13755
Lin Yang ₁ , Changdong Fan ₂ , Taipengfei Shu ₃ , Shujun Wang ₄

Affiliation

叉头盒 O3 (FOXO3) 转录因子参与正常、衰老和骨关节炎 (OA) 软骨的软骨稳态。目前，我们的目的是在体外和体内模型中研究FOXO3是否是安石榴苷预防IL-1β和TNF-α诱导的软骨细胞功能障碍的靶点。建立了软骨细胞功能障碍的细胞和小鼠模型，以确定从石榴中提取的可水解单宁安石榴苷的药理价值。使用蛋白质印迹分析细胞核和细胞质中的 FOXO3 蛋白水平。番红 O 染色用于评估 IL-1β 和 TNF-α 处理小鼠生长板的扩张和软骨细胞分化。在IL-1β和TNF-α处理的软骨细胞和小鼠中，IL-1β和TNF-α引起FOXO3的磷酸化和核质穿梭，以及细胞核中FOXO3表达水平的降低。然而，安石榴苷处理抑制了 FOXO3 磷酸化和细胞质转移。安石榴苷治疗可改善IL-1β和TNF-α诱导的软骨细胞生长抑制和凋亡以及生长板和肥厚区的异常扩张。此外，安石榴甙可以通过介导多个基因表达来维持软骨细胞的正常表型。通过保留 FOXO3 的转录活性，安石榴苷对 IL-1β 和 TNF-α 刺激的软骨细胞和软骨代谢紊乱显示出有益的作用。

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Punicalin alleviates TNF-α- and IL-1β-induced chondrocyte dysfunction and cartilage metabolism via mediating FOXO3 signaling axis

Forkhead box O3 (FOXO3) transcription factor is involved in chondral homeostasis of normal, aging and osteoarthritis (OA) cartilage. At present, we aimed to investigate whether FOXO3 is a target of punicalin to prevent IL-1β- and TNF-α-induced chondrocyte dysfunction in vitro and in vivo models. Cell and mouse models of chondrocyte dysfunction were established to determine the pharmacological value of hydrolyzable tannin, punicalin, which was extracted from the pomegranate. FOXO3 protein levels in the nucleus and cytoplasm were analysed using western blot. Safranine O staining was performed to evaluate the expansion of growth plate and chondrocyte differentiation in IL-1β- and TNF-α-treated mice. In IL-1β- and TNF-α-treated chondrocytes and mice, IL-1β and TNF-α evoked phosphorylation and nucleocytoplasmic shuttling of FOXO3, as well as reduced FOXO3 expression levels in the nucleus. However, punicalin treatment repressed FOXO3 phosphorylation and cytoplasmic transfer. Punicalin treatment improved IL-1β and TNF-α-induced growth inhibition and apoptosis of chondrocyte and the abnormal expansion of growth plate and hypertrophic zone. Moreover, punicalin could maintain the normal phenotype of chondrocyte via mediating multiple gene expression. Punicalin showed a beneficial effect on IL-1β- and TNF-α-stimulated chondrocytes and cartilaginous metabolic disorders via preserving the transcriptional activity of FOXO3.

更新日期：2021-05-11

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