<br>人体骨骼重塑过程中骨细胞网络与形成矿物之间的 3D 相互关系,Advanced Healthcare Materials

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人体骨骼重塑过程中骨细胞网络与形成矿物之间的 3D 相互关系
Advanced Healthcare Materials ( IF 10.0 ) Pub Date : 2021-05-08 , DOI: 10.1002/adhm.202100113
Mahdi Ayoubi _{1,

2} , Alexander F van Tol _{1,

2} , Richard Weinkamer ₁ , Paul Roschger ₃ , Peter C Brugger ₄ , Andrea Berzlanovich ₅ , Luca Bertinetti _{1,

6} , Andreas Roschger _{1,

7} , Peter Fratzl ₁

Affiliation

在骨骼重塑过程中，已知成骨细胞会沉积未矿化的胶原组织（类骨质），这些组织会在一段时间后矿化。一些成骨细胞分化成骨细胞，在骨的腔隙小管网络（LCN）内形成细胞网络。为了更深入地了解骨细胞在类骨细胞矿化过程中的潜在作用，使用聚焦离子束扫描电子显微镜（FIB-SEM）对骨形成位点的 9 个形成人类骨质进行三维成像。与之前的观察结果一致，发现矿物质浓度从中央 Haversian 管向预先存在的矿化骨骼逐渐增加。最有趣的是，在长度尺度上发现了一个小 100 多倍的类似特征，其中矿物浓度从 LCN 开始增加，在小管周围留下了一个几乎没有矿物的区域，其大小随着矿化的进行而减小。这表明 LCN 控制矿物形成，但不仅仅是通过矿化前体的扩散，这将导致 LCN 的矿物浓度持续降低。然而，该观察结果与来自 LCN 的前体和抑制剂进入骨基质的共扩散和反应是兼容的。

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3D Interrelationship between Osteocyte Network and Forming Mineral during Human Bone Remodeling

During bone remodeling, osteoblasts are known to deposit unmineralized collagenous tissue (osteoid), which mineralizes after some time lag. Some of the osteoblasts differentiate into osteocytes, forming a cell network within the lacunocanalicular network (LCN) of bone. To get more insight into the potential role of osteocytes in the mineralization process of osteoid, sites of bone formation are three-dimensionally imaged in nine forming human osteons using focused ion beam-scanning electron microscopy (FIB-SEM). In agreement with previous observations, the mineral concentration is found to gradually increase from the central Haversian canal toward pre-existing mineralized bone. Most interestingly, a similar feature is discovered on a length scale more than 100-times smaller, whereby mineral concentration increases from the LCN, leaving around the canaliculi a zone virtually free of mineral, the size of which decreases with progressing mineralization. This suggests that the LCN controls mineral formation but not just by diffusion of mineralization precursors, which would lead to a continuous decrease of mineral concentration from the LCN. The observation is, however, compatible with the codiffusion and reaction of precursors and inhibitors from the LCN into the bone matrix.

更新日期：2021-06-23

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