The E3 ligase XBAT35 mediates thermoresponsive hypocotyl growth by targeting ELF3 for degradation in Arabidopsis,Journal of Integrative Plant Biology

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The E3 ligase XBAT35 mediates thermoresponsive hypocotyl growth by targeting ELF3 for degradation in Arabidopsis
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2021-05-07 , DOI: 10.1111/jipb.13107
Lin-Lin Zhang ₁ , Wei Li ₁ , Ying-Ying Tian ₁ , Seth Jon Davis _{2,

3} , Jian-Xiang Liu ₁

Affiliation

Plants are capable of coordination of their growth and development with ambient temperatures. EARLY FLOWERING3 (ELF3), an essential component of the plant circadian clock, is also involved in ambient temperature sensing, as well as in inhibiting the expression and protein activity of the thermoresponsive regulator phytochrome interacting factor 4 (PIF4). The ELF3 activity is subjected to attenuation in response to warm temperature; however, how the protein level of ELF3 is regulated at warm temperature remains less understood. Here, we report that the E3 ligase XB3 ORTHOLOG 5 IN ARABIDOPSIS THALIANA, XBAT35, mediates ELF3 degradation. XBAT35 interacts with ELF3 and ubiquitinates ELF3. Loss-of-function mutation of XBAT35 increases the protein level of ELF3 and confers a short-hypocotyl phenotype under warm temperature conditions. Thus, our findings establish that XBAT35 mediates ELF3 degradation to lift the inhibition of ELF3 on PIF4 for promoting thermoresponsive hypocotyl growth in plants.

中文翻译：

E3 连接酶 XBAT35 通过靶向 ELF3 在拟南芥中降解来介导热响应性下胚轴生长

植物能够根据环境温度协调它们的生长和发育。EARLY FLOWERING3 (ELF3) 是植物生物钟的重要组成部分，也参与环境温度传感，以及抑制热响应调节剂光敏色素相互作用因子 4 (PIF4) 的表达和蛋白质活性。ELF3活性随着温度的升高而减弱；然而，在温暖的温度下如何调节 ELF3 的蛋白质水平仍然鲜为人知。在这里，我们报告了 E3 连接酶 XB3 ORTHOLOG 5 IN ARABIDOPSIS THALIANA，XBAT35，介导 ELF3 降解。XBAT35 与 ELF3 相互作用并泛素化 ELF3。XBAT35的功能丧失突变在温暖的温度条件下增加 ELF3 的蛋白质水平并赋予短下胚轴表型。因此，我们的研究结果表明 XBAT35 介导 ELF3 降解以解除 ELF3 对 PIF4 的抑制，从而促进植物中的热响应性下胚轴生长。

更新日期：2021-06-18

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