雀斑相关的肠沙门氏菌鼠伤寒沙门氏菌谱系对禽宿主的病理适应。,PLoS Pathogens

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雀斑相关的肠沙门氏菌鼠伤寒沙门氏菌谱系对禽宿主的病理适应。
PLoS Pathogens ( IF 5.5 ) Pub Date : 2021-03-19 , DOI: 10.1371/journal.ppat.1009451
Emiliano Cohen ₁ , Shalevet Azriel ₁ , Oren Auster _{1,

2,

3} , Adiv Gal ₄ , Carmel Zitronblat ₅ , Svetlana Mikhlin ₆ , Felix Scharte ₇ , Michael Hensel ₇ , Galia Rahav _{1,

2} , Ohad Gal-Mor _{1,

2,

3}

Affiliation

沙门氏菌是一种多样的细菌病原体，是人类和动物感染的主要原因。虽然许多 S. enterica 血清型具有广泛的宿主特异性，但几种特殊的病理类型已经适应于在一种或有限数量的宿主物种中定殖和引起疾病。定义沙门氏菌宿主特异性的潜在机制尚不清楚。在这里，我们介绍了从以色列的几只野生麻雀中分离出的单相 S. enterica 血清型鼠伤寒菌株的遗传分析、表型特征和毒力分析。全基因组测序及其基因组的完整组装展示了独特的遗传特征，包括 BTP1 原噬菌体的整合、毒力质粒的缺失、pSLT 和多个 T3SS-2 效应子（sseJ、steC、gogB、sseK2 和 sseK3）中的假基因积累), 过氧化氢酶 (katE)、连四硫酸盐呼吸 (ttrB) 和几种粘附/定植因子（lpfD、fimH、bigA、ratB、siiC 和 siiE）编码基因。相应地，该菌株表现出生物膜形成受损、氧化应激不耐受和非吞噬宿主细胞内的细胞内复制受损。此外，虽然该菌株在小鼠中显示出减弱的致病性，但它具有高毒力并在鸟类宿主中引起炎症性疾病。总体而言，我们的研究结果证明了一个被忽视的 S. Typhimurium 麻雀相关谱系的独特表型谱和基因组成，并呈现出可能有助于其对雀类鸟类的病理适应的独特遗传特征。siiC 和 siiE) 编码的基因。相应地，该菌株表现出生物膜形成受损、氧化应激不耐受和非吞噬宿主细胞内的细胞内复制受损。此外，虽然该菌株在小鼠中显示出减弱的致病性，但它具有高毒力并在鸟类宿主中引起炎症性疾病。总体而言，我们的研究结果证明了一个被忽视的 S. Typhimurium 麻雀相关谱系的独特表型谱和基因组成，并呈现出可能有助于其对雀类鸟类的病理适应的独特遗传特征。siiC 和 siiE) 编码的基因。相应地，该菌株表现出生物膜形成受损、氧化应激不耐受和非吞噬宿主细胞内的细胞内复制受损。此外，虽然该菌株在小鼠中显示出减弱的致病性，但它具有高毒力并在鸟类宿主中引起炎症性疾病。总体而言，我们的研究结果证明了一个被忽视的 S. Typhimurium 麻雀相关谱系的独特表型谱和基因组成，并呈现出可能有助于其对雀类鸟类的病理适应的独特遗传特征。虽然该菌株在小鼠中显示出减弱的致病性，但它具有高毒力并在鸟类宿主中引起炎症性疾病。总体而言，我们的研究结果证明了一个被忽视的 S. Typhimurium 麻雀相关谱系的独特表型谱和基因组成，并呈现出可能有助于其对雀类鸟类的病理适应的独特遗传特征。虽然该菌株在小鼠中显示出减弱的致病性，但它具有高毒力并在鸟类宿主中引起炎症性疾病。总体而言，我们的研究结果证明了一个被忽视的 S. Typhimurium 麻雀相关谱系的独特表型谱和基因组成，并呈现出可能有助于其对雀类鸟类的病理适应的独特遗传特征。

"点击查看英文标题和摘要"

Pathoadaptation of the passerine-associated Salmonella enterica serovar Typhimurium lineage to the avian host.

Salmonella enterica is a diverse bacterial pathogen and a primary cause of human and animal infections. While many S. enterica serovars present a broad host-specificity, several specialized pathotypes have been adapted to colonize and cause disease in one or limited numbers of host species. The underlying mechanisms defining Salmonella host-specificity are far from understood. Here, we present genetic analysis, phenotypic characterization and virulence profiling of a monophasic S. enterica serovar Typhimurium strain that was isolated from several wild sparrows in Israel. Whole genome sequencing and complete assembly of its genome demonstrate a unique genetic signature that includes the integration of the BTP1 prophage, loss of the virulence plasmid, pSLT and pseudogene accumulation in multiple T3SS-2 effectors (sseJ, steC, gogB, sseK2, and sseK3), catalase (katE), tetrathionate respiration (ttrB) and several adhesion/ colonization factors (lpfD, fimH, bigA, ratB, siiC and siiE) encoded genes. Correspondingly, this strain demonstrates impaired biofilm formation, intolerance to oxidative stress and compromised intracellular replication within non-phagocytic host cells. Moreover, while this strain showed attenuated pathogenicity in the mouse, it was highly virulent and caused an inflammatory disease in an avian host. Overall, our findings demonstrate a unique phenotypic profile and genetic makeup of an overlooked S. Typhimurium sparrow-associated lineage and present distinct genetic signatures that are likely to contribute to its pathoadaptation to passerine birds.

更新日期：2021-03-19

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