The prevalence and destructiveness of anthracnose, caused by Colletotrichum scovillei, in pepper production regions seriously affects pepper yield and quality. Mefentrifluconazole, the first of the isopropanol-azole subgroup of triazole fungicides, was introduced for the control of pepper anthracnose. However, the growth characteristics of pepper fruit and rapid spread of anthracnose suggest that the fungicide application method must be optimized to enhance fungicide efficacy. The sensitivity of C. scovillei to mefentrifluconazole was determined by mycelial growth and germ tube elongation assays using 157 single-spore isolates with mean 50% effective concentration values of 0.462 ± 0.138 and 0.359 ± 0.263 mg/liter, respectively. The in vivo data also showed that mefentrifluconazole had favorable protective and curative effects against pepper anthracnose. Mefentrifluconazole significantly affected C. scovillei infection on pepper by reducing appressorium formation and sporulation, shriveling spores and germ tubes, and causing the abnormal development of appressoria and conidiophores. Mefentrifluconazole could move acropetally, horizontally, and basipetally in pepper plants. Compared with a knapsack sprayer, mefentrifluconazole applied by mist sprayer exhibited significantly better activity against pepper anthracnose. Additionally, as the spray volume increased from 45 to 150 liters/ha, the control efficacy of mefentrifluconazole first increased and then tended to be steady, with an optimal spray volume of 90 liters/ha. The difference in disease control efficacy was related to the deposition and droplet distribution of mefentrifluconazole on the pepper fruit. These results provide scientific guidance for the application of mefentrifluconazole in pepper fields and improved fungicide utilization.

中文翻译：

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新型杀菌剂甲芬氟康唑对炭疽菌的活性。

辣椒产区炭疽菌引起的炭疽病流行和破坏性严重影响辣椒的产量和质量。引入了三唑类杀真菌剂的异丙醇-吡唑亚组的第一个甲芬氟康唑，用于控制辣椒炭疽病。然而，辣椒果实的生长特性和炭疽病的迅速传播表明必须优化杀菌剂的施用方法以提高杀菌剂的功效。通过使用157种单孢分离株的菌丝体生长和胚管伸长测定法确定了斯科氏梭菌对甲芬氟康唑的敏感性，其50％有效浓度平均值分别为0.462±0.138和0.359±0.263 mg / L。体内数据还表明，甲芬三氟康唑对辣椒炭疽病具有良好的保护和治疗作用。甲芬氟康唑可通过减少食堂的形成和孢子形成，减少孢子和生殖管的收缩以及引起食堂和分生孢子的异常发展来显着影响辣椒上的斯科氏菌感染。甲芬氟康唑可以在辣椒植物中向顶，水平和立足移动。与背负式喷雾器相比，喷雾喷雾器施用的甲芬特氟康唑对辣椒炭疽病具有明显更好的活性。此外，随着喷雾量从45升/公顷增加到150升/公顷，甲芬氟康唑的防治效果首先增加，然后趋于稳定，最佳喷雾量为90升/公顷。疾病控制功效的差异与胡椒果实上甲芬氟康唑的沉积和液滴分布有关。这些结果为在农药领域中使用甲芬特氟康唑和提高杀菌剂的利用提供了科学指导。