Background: Combination of different chemotherapy drugs and nanoparticles as a carrier has shown promising delivery system in cancer treatment. Doxorubicin is considered a potent anticancer drug. However, its off-target activities and possible side effects make its use limited. Recently, in the field of nanomedicine, different nanoconjugates have been developed as a unique platform for the delivery of therapeutic drugs.

Aims: The aim of the present study is to evaluate the best possible combination for efficient delivery of DOX with combination of gold, silver and zinc oxide nanoparticles to target site against carbon tetrachloride induced rat hepatotoxicity.

Methods: Effect of different conjugates administrated for 14 consecutive days was evaluated.

Results: In comparison to DOX, Au:DOX, ZnoO:DOX and Ag:DOX showed less sign of liver fibrosis as evaluated by serum enzymes and histopathological analysis. However, among all the conjugates, Ag: DOX conjugate showed the most significant results. The serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase values were (111.2 ± 38.21, 323.2 ± 46.88 and 303.6 ± 73.80 respectively) very close to control group (72.2 ± 19.41, 368 ± 59.78 and 259.4 ± 61.54 respectively).

Conclusion: Our results demonstrated that Ag: DOX may exhibit hepato-protective activity against CCl4 induced liver damage.

背景：不同化疗药物和纳米颗粒作为载体的组合在癌症治疗中显示出有前景的递送系统。阿霉素被认为是一种有效的抗癌药物。然而，其脱靶活性和可能的​​副作用使其使用受到限制。最近，在纳米医学领域，已开发出不同的纳米缀合物作为治疗药物递送的独特平台。

目的：本研究的目的是评估将 DOX 与金、银和氧化锌纳米颗粒的组合有效递送至靶点以对抗四氯化碳诱导的大鼠肝毒性的最佳组合。

方法：评估了连续 14 天施用的不同缀合物的效果。

结果：与 DOX 相比，通过血清酶和组织病理学分析评估，Au:DOX、ZnoO:DOX 和 Ag:DOX 显示出较少的肝纤维化迹象。然而，在所有偶联物中，Ag:DOX 偶联物显示出最显着的结果。血清丙氨酸转氨酶、天冬氨酸转氨酶、碱性磷酸酶值分别为（111.2±38.21、323.2±46.88和303.6±73.80）非常接近对照组（分别为72.2±19.41、368±59.54和59.78）。

结论：我们的结果表明，Ag: DOX 可能对 CCl4 引起的肝损伤具有保肝活性。