Candida albicans is an opportunistic fungal human pathogen that has been causing an increasing number of deaths each year. Due to the widespread use of broad-spectrum antibiotics and immunosuppressants, C. albicans resistance to these therapies has increased. Thus, natural plant inhibitors are being investigated for treating C. albicans infections. Schinifoline is a 4-quinolinone alkaloid with antibacterial, insecticidal, antitumor, and other biological activities. Here, we explored the effects of schinifoline on C. albicans in C. elegans and extracted RNA from uninfected C. elegans, C. elegans infected with C. albicans, and C. elegans infected with C. albicans and treated with 100 mg/l schinifoline. Our results showed that there were significant differences among the three groups. The GO and KEGG pathway analysis suggested that the pathogenicity of C. albicans to C. elegans was caused by abnormal protein function. Schinifoline regulates lysosomal pathway related genes that accelerate the metabolism and degradation of abnormal proteins, thereby inhibiting the negative effects of C. albicans in vivo. These findings advance our understanding of the molecular mechanisms underlying schinifoline inhibition of C. albicans.

白色念珠菌是一种机会性真菌人类病原体，每年造成越来越多的死亡。由于广谱抗生素和免疫抑制剂的广泛使用，白色念珠菌对这些疗法的耐药性有所增加。因此，正在研究用于治疗白色念珠菌感染的天然植物抑制剂。Schinifoline 是一种 4-喹啉酮类生物碱，具有抗菌、杀虫、抗肿瘤和其他生物活性。在这里，我们探讨schinifoline对影响白色念珠菌在秀丽隐杆线虫的感染和提取RNA线虫，线虫感染了白色念珠菌，和感染了白色念珠菌并用 100 mg/l schinifoline 处理的秀丽隐杆线虫。我们的结果表明，三组之间存在显着差异。GO和KEGG通路分析表明白色念珠菌对秀丽隐杆线虫的致病性是由蛋白质功能异常引起的。Schinifoline 调节溶酶体通路相关基因，加速异常蛋白质的代谢和降解，从而抑制体内白色念珠菌的负面影响。这些发现促进了我们对白色念珠菌抑制 schinifoline 的分子机制的理解。