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Capsulation of AuNCs with AIE Effect into Metal–Organic Framework for the Marriage of a Fluorescence and Colorimetric Biosensor to Detect Organophosphorus Pesticides
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-05-06 , DOI: 10.1021/acs.analchem.1c00616 Yue Cai 1 , Hongshuai Zhu 1, 2 , Weichi Zhou 3 , Ziyin Qiu 1 , Congcong Chen 1 , Aori Qileng 1, 2 , Kangshun Li 3 , Yingju Liu 1, 2
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-05-06 , DOI: 10.1021/acs.analchem.1c00616 Yue Cai 1 , Hongshuai Zhu 1, 2 , Weichi Zhou 3 , Ziyin Qiu 1 , Congcong Chen 1 , Aori Qileng 1, 2 , Kangshun Li 3 , Yingju Liu 1, 2
Affiliation
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Organophosphorus pesticides (OPs) can inhibit the activity of acetylcholinesterase (AChE) to induce neurological diseases. It is significant to exploit a rapid and sensitive strategy to monitor OPs. Here, a metal–organic framework (MOF) acted as a carrier to encapsulate AuNCs, which can limit the molecular motion of AuNCs, trigger the aggregation-induced emission (AIE) effect, and exhibit a strong fluorescence with a fluorescence lifetime and quantum yield of 6.83 μs and 4.63%, respectively. Then, the marriage of fluorescence and colorimetric signals was realized on the basis of the dual function of the enzymolysis product from AChE and choline oxidase (CHO) on AuNCs@ZIF-8. First, it can decompose ZIF-8 to weaken the restraint on AuNCs, and thus the fluorescence receded. Second, it can be used as a substrate for the peroxidase mimics of the released AuNCs to oxidize 3,3′,5,5′-tetramethylbenzidine (TMB) and a visible blue appeared. Thus, on the basis of the inhibition of AChE activity by OPs, a fluorescence–colorimetric dual-signal biosensor was established. In addition, colorimetric paper strips were exploited to realize a visual semiquantitative detection, and a smartphone APP was developed to make the visualization results more precise and realize real-time supervision of pesticide contamination.
中文翻译：
将具有AIE效应的AuNCs封装到金属有机框架中，以结合荧光和比色生物传感器来检测有机磷农药。
有机磷农药（OPs）可以抑制乙酰胆碱酯酶（AChE）的活性，从而诱发神经系统疾病。开发一种快速而敏感的策略来监视OP具有重要意义。在这里，金属有机骨架（MOF）充当了封装AuNCs的载体，这可以限制AuNCs的分子运动，触发聚集诱导发射（AIE）效应，并显示出具有荧光寿命和量子产率的强荧光。分别为6.83μs和4.63％。然后，基于AChE和胆碱氧化酶（CHO）在AuNCs @ ZIF-8上的酶解产物的双重功能，实现了荧光和比色信号的结合。首先，它可以分解ZIF-8以减弱对AuNCs的抑制，从而使荧光减弱。第二，它可用作释放的AuNCs过氧化物酶模拟物的底物，以氧化3,3'，5,5'-四甲基联苯胺（TMB），并出现可见的蓝色。因此，基于OP对AChE活性的抑制作用，建立了荧光比色双信号生物传感器。此外，利用比色纸条实现了视觉半定量检测，并开发了智能手机APP以使可视化结果更加精确并实现对农药污染的实时监控。
更新日期：2021-05-18
中文翻译：
将具有AIE效应的AuNCs封装到金属有机框架中，以结合荧光和比色生物传感器来检测有机磷农药。
有机磷农药（OPs）可以抑制乙酰胆碱酯酶（AChE）的活性，从而诱发神经系统疾病。开发一种快速而敏感的策略来监视OP具有重要意义。在这里，金属有机骨架（MOF）充当了封装AuNCs的载体，这可以限制AuNCs的分子运动，触发聚集诱导发射（AIE）效应，并显示出具有荧光寿命和量子产率的强荧光。分别为6.83μs和4.63％。然后，基于AChE和胆碱氧化酶（CHO）在AuNCs @ ZIF-8上的酶解产物的双重功能，实现了荧光和比色信号的结合。首先，它可以分解ZIF-8以减弱对AuNCs的抑制，从而使荧光减弱。第二，它可用作释放的AuNCs过氧化物酶模拟物的底物，以氧化3,3'，5,5'-四甲基联苯胺（TMB），并出现可见的蓝色。因此，基于OP对AChE活性的抑制作用，建立了荧光比色双信号生物传感器。此外，利用比色纸条实现了视觉半定量检测，并开发了智能手机APP以使可视化结果更加精确并实现对农药污染的实时监控。
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