免疫球蛋白超家族成员 10 是一种新型的乳腺癌预后生物标志物,PeerJ

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免疫球蛋白超家族成员 10 是一种新型的乳腺癌预后生物标志物
PeerJ ( IF 2.3 ) Pub Date : 2020-10-21 , DOI: 10.7717/peerj.10128
Mengxue Wang ₁ , Meng Dai ₂ , Yu-Shen Wu ₁ , Ziying Yi ₁ , Yunhai Li ₃ , Guosheng Ren ₁

Affiliation

背景免疫球蛋白超家族成员 10 (IGSF10) 是免疫球蛋白超家族的成员，在胆囊和卵巢中均以高水平表达。目前，IGSF10在乳腺癌中的作用和可能的机制尚不清楚。方法应用实时定量聚合酶链反应（qRT-PCR）和免疫组化（IHC）检测乳腺癌细胞和组织中IGSF10的表达。我们在癌症基因组图谱（TCGA）中收集了 700 名乳腺癌患者的临床信息，并分析了 IGSF10 表达与这些患者的临床病理特征和生存结果之间的关系。通过进行基因集富集分析 (GSEA) 探索了与 IGSF10 在乳腺癌中相关的潜在机制和途径。结果根据TCGA数据、qRT-PCR和IHC实验，乳腺癌组织中IGSF10 mRNA和蛋白水平显着降低。IGSF10 表达与年龄、肿瘤大小和肿瘤分期显着相关。此外，根据生存分析，较短的总生存期 (OS) 和无复发生存期 (RFS) 与较低的 IGSF10 表达相关。多变量分析确定 IGSF10 是 OS（风险比 (HR) = 1.793, 95% 置信区间 (CI) [1.141–2.815], P = 0.011）和 RFS（HR = 2.298, 95% CI）的独立预后因素[1.317–4.010]，P = 0.003) 的乳腺癌患者。基于 GSEA，IGSF10 参与 DNA 修复、细胞周期和糖酵解。IGSF10 还与 PI3K/Akt/mTOR 和 mTORC1 信号通路相关。结论本研究首次揭示了 IGSF10 表达与乳腺癌肿瘤发生的明确关系。因此，需要进一步的研究来了解 IGSF10 在乳腺癌中的作用机制。

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Immunoglobulin superfamily member 10 is a novel prognostic biomarker for breast cancer

Background Immunoglobulin superfamily member 10 (IGSF10) is a member of the immunoglobulin superfamily that is expressed at high levels in both the gallbladder and ovary. Currently, the role and possible mechanism of IGSF10 in breast cancer remain unclear. Method By applying real-time quantitative polymerase chain reaction (qRT-PCR) and immunohistochemistry (IHC), the expression of IGSF10 in breast cancer cells and tissues was detected. We collected the clinical information from 700 patients with breast cancer in The Cancer Genome Atlas (TCGA), and analyzed the relationship between IGSF10 expression and the clinicopathological features and survival outcomes of these patients. The potential mechanisms and pathways associated with IGSF10 in breast cancer were explored by performing a gene set enrichment analysis (GSEA). Results According to TCGA data, qRT-PCR and IHC experiments, levels of the IGSF10 mRNA and protein were significantly decreased in breast cancer tissues. IGSF10 expression was significantly correlated with age, tumor size, and tumor stage. Moreover, shorter overall survival (OS) and relapse-free survival (RFS) correlated with lower IGSF10 expression, according to the survival analysis. The multivariate analysis identified that IGSF10 as an independent prognostic factor for the OS (hazard ratio (HR) = 1.793, 95% confidence interval (CI) [1.141–2.815], P = 0.011) and RFS (HR = 2.298, 95% CI [1.317–4.010], P = 0.003) of patients with breast cancer. Based on the GSEA, IGSF10 was involved in DNA repair, cell cycle, and glycolysis. IGSF10 was also associated with the PI3K/Akt/mTOR and mTORC1 signaling pathways. Conclusions This study revealed a clear relationship between IGSF10 expression and the tumorigenesis of breast cancer for the first time. Therefore, further studies are needed to understand the mechanism of IGSF10 in breast cancer.

更新日期：2020-10-21

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