This study was conducted to investigate the hypothesis that the beneficial metabolic effects of dietary fiber, konjac glucomannan (KGM), related with in vivo digestion might be altered if the complicated food matrix was taken into consideration. A diet of precooked noodles (PN), as widely produced and consumed in Asia, was used to simulate an actual food context. Assays were conducted with male Wistar rats (n = 80); the rats were divided into five groups and fed with either PN (control), PN supplemented with medium-dose KGM (MK), precooked low-dose KGM-supplemented noodles (LKD), precooked medium-dose KGM-supplemented noodles (MKD) or precooked high-dose KGM supplemented noodles (HKD). The time-dependent changes in blood glucose and the sensitivity to insulin after intragastric administration were determined to evaluate the postprandial glycemic response. The activity of intestinal Na+-K+-ATPase and the levels of gut hormones including motilin, cholecystokin, GLP-1, and orexin were also determined to provide insights into the function of gastrointestinal motion and after-meal hormonal feedback in each group. The noodles-based KGM showed much more efficacy in sustaining glucose homeostasis compared with KGM supplemented in a diet of noodles, indicating there might be potential long-term health outcomes of satiety and energy balance using noodles-based KGM. The postprandial glycemia was largely moderated by LKD and MKD. Despite the significant reduction in the production of glucose, MKD caused insensitivity to insulin–blood glucose regulation and a rapid gut negative feedback following a severe blood glucose fluctuation. In conclusion, the health-promoting benefits of KGM supplements on glycemic response highly depend on the type of matrix and the dose of KGM.

中文翻译：

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面条魔芋葡甘聚糖对大鼠胃肠道和代谢的影响

本研究的目的是调查这样的假设：如果考虑到复杂的食物基质，膳食纤维、魔芋葡甘聚糖 (KGM) 与体内消化相关的有益代谢作用可能会改变。在亚洲广泛生产和消费的预煮面条（PN）饮食被用来模拟实际的食物环境。使用雄性 Wistar 大鼠 (n = 80) 进行测定；将大鼠分为五组，分别喂食PN（对照组）、PN中添加中剂量KGM（MK）、预煮低剂量KGM面条（LKD）、预煮中剂量KGM面条（MKD）或预先煮熟的高剂量KGM添加面条（港元）。测定胃内给药后血糖随时间的变化和对胰岛素的敏感性，以评估餐后血糖反应。还测定了肠道Na+-K+-ATP酶的活性和肠道激素（包括胃动素、胆囊素、GLP-1和食欲素）的水平，以深入了解每组胃肠运动的功能和餐后激素反馈。与在面条饮食中补充的 KGM 相比，基于面条的 KGM 在维持葡萄糖稳态方面表现出更强的功效，这表明使用基于面条的 KGM 可能具有饱腹感和能量平衡的潜在长期健康结果。LKD 和 MKD 很大程度上缓解了餐后血糖。尽管葡萄糖的产生显着减少，MKD 仍导致对胰岛素-血糖调节不敏感，并在严重的血糖波动后快速产生肠道负反馈。总之，魔芋葡甘聚糖补充剂对血糖反应的健康促进作用很大程度上取决于基质的类型和魔芋葡甘聚糖的剂量。