Intra-Operative Bioprinting of Hard, Soft, and Hard/Soft Composite Tissues for Craniomaxillofacial Reconstruction,Advanced Functional Materials

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Intra-Operative Bioprinting of Hard, Soft, and Hard/Soft Composite Tissues for Craniomaxillofacial Reconstruction
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2021-04-22 , DOI: 10.1002/adfm.202010858
Kazim K Moncal _{1,

2} , Hemanth Gudapati _{1,

2} , Kevin P Godzik ₃ , Dong N Heo ₄ , Youngnam Kang ₁ , Elias Rizk ₅ , Dino J Ravnic ₆ , Hwabok Wee ₁ , David F Pepley ₇ , Veli Ozbolat ₈ , Gregory S Lewis ₁ , Jason Z Moore ₃ , Ryan R Driskell ₉ , Thomas D Samson ₅ , Ibrahim T Ozbolat ₁

Affiliation

Reconstruction of complex craniomaxillofacial (CMF) defects is challenging due to the highly organized layering of multiple tissue types. Such compartmentalization necessitates the precise and effective use of cells and other biologics to recapitulate the native tissue anatomy. In this study, intra-operative bioprinting (IOB) of different CMF tissues, including bone, skin, and composite (hard/soft) tissues, is demonstrated directly on rats in a surgical setting. A novel extrudable osteogenic hard tissue ink is introduced, which induced substantial bone regeneration, with ≈80% bone coverage area of calvarial defects in 6 weeks. Using droplet-based bioprinting, the soft tissue ink accelerated the reconstruction of full-thickness skin defects and facilitated up to 60% wound closure in 6 days. Most importantly, the use of a hybrid IOB approach is unveiled to reconstitute hard/soft composite tissues in a stratified arrangement with controlled spatial bioink deposition conforming the shape of a new composite defect model, which resulted in ≈80% skin wound closure in 10 days and 50% bone coverage area at Week 6. The presented approach will be absolutely unique in the clinical realm of CMF defects and will have a significant impact on translating bioprinting technologies into the clinic in the future.

中文翻译：

用于颅颌面重建的硬、软和硬/软复合组织的术中生物打印

由于多种组织类型的高度组织化分层，复杂颅颌面（CMF）缺损的重建具有挑战性。这种区室化需要精确有效地使用细胞和其他生物制剂来重现天然组织解剖结构。在这项研究中，直接在手术环境中的大鼠身上演示了不同 CMF 组织（包括骨、皮肤和复合（硬/软）组织）的术中生物打印 (IOB)。引入了一种新型可挤出成骨硬组织墨水，可诱导大量骨再生，在 6 周内颅骨缺损的骨覆盖面积约 80%。使用基于液滴的生物打印，软组织墨水加速了全层皮肤缺损的重建，并在 6 天内促进了高达 60% 的伤口闭合。最重要的是，使用混合 IOB 方法以分层排列的方式重建硬/软复合组织，并通过受控的空间生物墨水沉积来符合新的复合缺陷模型的形状，从而在 10 天内实现约 80% 的皮肤伤口闭合第 6 周骨覆盖面积达到 50%。所提出的方法在 CMF 缺陷的临床领域绝对是独一无二的，并将对未来将生物打印技术转化为临床产生重大影响。

更新日期：2021-04-22

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