T–2 toxin is one of class A trichothecene mycotoxins produced by Fusarium, presenting genotoxic, cytotoxicity, and immunotoxicity for animals and humans. Therefore, It is urgent to establish a rapid test method with high sensitivity, good selectivity and reliability. In this research, by adjusting the synthesis conditions, a kind of NH₂–UiO–66 with high quenching efficiency was screened out. On this basis, we constructed a novel fluorescence sensor via Cy3–labeled aptamer (Cy3–aptamer). With the help of π–π interaction, hydrogen bond and coordination, NH₂–UiO–66 could adsorb and quench the fluorescence of Cy3–aptamer based on FRET and PET. In the presence of T–2 toxin, it recognized and bound to Cy3–aptamer, leading to the disintegration of the NH₂–UiO–66/Cy3–aptamer compound. As the energy transfer process was blocked, the fluorescence intensity was restored, enabling a highly sensitive response to T–2 toxin. There was a good linear correlation between fluorescence intensity and T–2 toxin concentration in the range of 0.5–100 ng ml ⁻¹. The LOD of this fluorescence aptasensor was 0.239 ng ml⁻¹ (S/N = 3). Besides, the recoveries of milk and beer were 89.86–108.99% (RSD = 2.0–2.6%) and 92.31–111.51% (RSD = 2.3–2.9%), respectively. The fluorescence aptasensor exhibited advantages of excellent analytical performance, convenient operation procedure and good selectivity. Predictably, the aptasensor was supposed to detect antibiotics and other pollutants, describing an intriguing blueprint and potential application prospect in food safety, biochemical sensing and environmental conservation.

T-2毒素是镰刀菌（Fusarium）生产的A类天花粉真菌毒素，对动物和人类均具有遗传毒性，细胞毒性和免疫毒性。因此，迫切需要建立一种灵敏度高，选择性好，可靠性高的快速检测方法。本研究通过调节合成条件，筛选出一种淬灭效率高的NH ₂ -UiO-66。在此基础上，我们通过Cy3标记的适体（Cy3-适体）构建了新型荧光传感器。借助π-π相互作用，氢键和配位，NH ₂ -UiO-66可以吸附和猝灭基于FRET和PET的Cy3-适体的荧光。在存在T–2毒素的情况下，它会识别并与Cy3–适体结合，从而导致NH ₂分解–UiO–66 / Cy3–适体化合物。由于能量转移过程受阻，荧光强度得以恢复，从而对T-2毒素产生了高度敏感的反应。荧光强度与T–2毒素浓度在0.5–100 ng ml ^-1范围内具有良好的线性关系。该荧光适体传感器的LOD为0.239 ng ml ^-1（S / N = 3）。此外，牛奶和啤酒的回收率分别为89.86–108.99％（RSD = 2.0–2.6％）和92.31–111.51％（RSD = 2.3–2.9％）。荧光适体传感器具有分析性能优良，操作步骤方便，选择性好等优点。aptasensor可以预测地检测出抗生素和其他污染物，从而描述了一个引人入胜的蓝图，并在食品安全，生化检测和环境保护方面具有潜在的应用前景。