Tissue-based assay (TBA) is a widely-used method to detect neural autoantibodies, but the diagnostic accuracy for autoimmune encephalitis (AE) has not yet been adequately measured. We retrospectively evaluated the sensitivity and specificity of an indirect immunofluorescence TBA (IIF-TBA) in 159 patients with suspected AE. Serum and cerebrospinal fluid (CSF) specimens were collected and tested from December 2012 to September 2020. In the paired sample analysis, serum testing showed higher sensitivity than CSF, while the latter had higher specificity. Based on these results, we clarify the advantages of using a TBA as the principal screening method for patients with suspected AE.

基于组织的测定 (TBA) 是一种广泛使用的检测神经自身抗体的方法，但尚未充分测量自身免疫性脑炎 (AE) 的诊断准确性。我们回顾性评估了 159 名疑似 AE 患者间接免疫荧光 TBA (IIF-TBA) 的敏感性和特异性。血清和脑脊液（CSF）标本采集时间为2012年12月至2020年9月。在配对样本分析中，血清检测显示出比CSF更高的敏感性，而后者具有更高的特异性。基于这些结果，我们阐明了使用 TBA 作为疑似 AE 患者的主要筛查方法的优势。