Nanozymes have become a new generation of antibiotics with exciting broad-spectrum antibacterial properties and negligible biological toxicity. However, their inherent low catalytic activity limits their antibacterial properties. Herein, Cu single-atom sites/N doped porous carbon (Cu SASs/NPC) is successfully constructed for photothermal-catalytic antibacterial treatment by a pyrolysis-etching-adsorption-pyrolysis (PEAP) strategy. Cu SASs/NPC have stronger peroxidase-like catalytic activity, glutathione (GSH)-depleting function, and photothermal property compared with non-Cu-doped NPC, indicating that Cu doping significantly improves the catalytic performance of nanozymes. Cu SASs/NPC can effectively induce peroxidase-like activity in the presence of H₂O₂, thereby generating a large amount of hydroxyl radicals (•OH), which have a certain killing effect on bacteria and make bacteria more susceptible to temperature. The introduction of near-infrared (NIR) light can generate hyperthermia to fight bacteria, and enhance the peroxidase-like catalytic activity, thereby generating additional •OH to destroy bacteria. Interestingly, Cu SASs/NPC can act as GSH peroxidase (GSH-Px)-like nanozymes, which can deplete GSH in bacteria, thereby significantly improving the sterilization effect. PTT-catalytic synergistic antibacterial strategy produces almost 100% antibacterial efficiency against Escherichia coli (E. coli) and methicillin-resistant Staphylococcus aureus (MRSA). In vivo experiments show a better PTT-catalytic synergistic therapeutic performance on MRSA-infected mouse wounds. Overall, our work highlights the wide antibacterial and anti-infective bio-applications of Cu single-atom-containing catalysts.

纳米酶已成为新一代抗生素，具有令人兴奋的广谱抗菌特性和可忽略不计的生物毒性。然而，它们固有的低催化活性限制了它们的抗菌性能。在此，通过热解-蚀刻-吸附-热解（PEAP）策略成功构建了铜单原子位点/氮掺杂多孔碳（Cu SASs/NPC），用于光热催化抗菌处理。与未掺杂Cu的NPC相比，Cu SASs/NPC具有更强的类过氧化物酶催化活性、谷胱甘肽（GSH）消耗功能和光热性能，表明Cu掺杂显着提高了纳米酶的催化性能。 Cu SASs/NPC在H ₂ O ₂存在下能有效诱导类过氧化物酶活性，从而产生大量的羟基自由基（•OH），对细菌有一定的杀伤作用，并使细菌对温度更加敏感。引入近红外（NIR）光可以产生高温来对抗细菌，并​​增强类过氧化物酶的催化活性，从而产生额外的·OH来消灭细菌。有趣的是，Cu SASs/NPC可以作为GSH过氧化物酶（GSH-Px）类纳米酶，可以消耗细菌中的GSH，从而显着提高灭菌效果。 PTT催化协同抗菌策略对大肠杆菌（ E. coli ）和耐甲氧西林金黄色葡萄球菌（ MRSA ）产生几乎100%的抗菌效率。体内实验显示对MRSA感染的小鼠伤口有更好的PTT催化协同治疗性能。 总的来说，我们的工作强调了含铜单原子催化剂的广泛抗菌和抗感染生物应用。