Evaluation of tissue integration of injectable, cell-laden hydrogels of cocultures of mesenchymal stem cells and articular chondrocytes with an ex vivo cartilage explant model,Biotechnology and Bioengineering

当前位置： X-MOL 学术 › Biotechnol. Bioeng. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Evaluation of tissue integration of injectable, cell-laden hydrogels of cocultures of mesenchymal stem cells and articular chondrocytes with an ex vivo cartilage explant model
Biotechnology and Bioengineering ( IF 3.5 ) Pub Date : 2021-04-29 , DOI: 10.1002/bit.27804
Yu Seon Kim ₁ , Shail M Mehta ₁ , Jason L Guo ₁ , Hannah A Pearce ₁ , Brandon T Smith ₁ , Emma Watson ₁ , Gerry L Koons ₁ , Adam M Navara ₁ , Johnny Lam ₂ , K Jane Grande-Allen ₁ , Antonios G Mikos ₁

Affiliation

This study investigated the chondrogenic activity of encapsulated mesenchymal stem cells (MSCs) and articular chondrocytes (ACs) and its impact on the mechanical properties of injectable poly(N-isopropylacrylamide)-based dual-network hydrogels loaded with poly( l -lysine) (PLL). To this effect, an ex vivo study model was employed to assess the behavior of the injected hydrogels—specifically, their surface stiffness and integration strength with the surrounding cartilage. The highest chondrogenic activity was observed from AC-encapsulated hydrogels, while the effect of PLL on MSC chondrogenesis was not apparent from biochemical analyses. Mechanical testing showed that there were no significant differences in either surface stiffness or integration strength among the different study groups. Altogether, the results suggest that the ex vivo model can allow further understanding of the relationship between biochemical changes within the hydrogel and their impact on the hydrogel's mechanical properties.

中文翻译：

使用离体软骨外植体模型评估间充质干细胞和关节软骨细胞共培养物的可注射、充满细胞的水凝胶的组织整合

本研究调查了封装的间充质干细胞（MSC）和关节软骨细胞（AC）的软骨形成活性及其对可注射聚（ N-异丙基丙烯酰胺）负载聚（L-赖氨酸）双网络水凝胶机械性能的影响。锁相环）。为此，采用离体研究模型来评估注射水凝胶的行为，特别是它们的表面硬度和与周围软骨的整合强度。从 AC 封装的水凝胶中观察到最高的软骨形成活性，而从生化分析来看 PLL 对 MSC 软骨形成的影响并不明显。机械测试表明，不同研究组之间的表面刚度或集成强度没有显着差异。总而言之，结果表明离体模型可以进一步了解水凝胶内的生化变化及其对水凝胶机械性能的影响之间的关系。

更新日期：2021-04-29

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南