Highly enriched active dechlorinating cultures are important in advancing microbial remediation technology. This study attempted to enrich a rapid perchloroethene (PCE) dechlorinating culture via magnetic nanoparticle-mediated isolation (MMI). MMI is a novel method that can separate the fast-growing and slow-growing population in a microbial community without labelling. In the MMI process, PCE dechlorination was enhanced but the subsequent trichloroethene (TCE) dechlorination was inhibited, with TCE cumulative rate reached up to 80.6% within 70 days. Meanwhile, the microbial community was also changed, with fast-growing genera like Dehalobacterium and Petrimonas enriched, and slow-growing Methanosarcina almost ruled out. Relative abundances of several major genera including Petrimonas and Methanosarcina were positively related to TCE dechlorination rate and the relative abundance of Dehalococcoides. On the other hand, Dehalobacterium was negatively related to TCE dechlorination rate and Dehalococcoides abundance, suggesting potential competition between Dehalobacterium and Dehalococcoides. The regrowth of Methanosarcina coupled well with the recovery of TCE dechlorination capacity, which implied the important role of methanogens in TCE dechlorination. Via MMI method, a simpler but more active microbial consortium could be established to enhance PCE remediation efficiency. Methanogens may act as the indicators or biomarkers for TCE dechlorination, suggesting that methanogenic activity should also be monitored when enriching dechlorination cultures and remediating PCE contaminated sites.

Capsule

A rapid perchloroethene dechlorinator was gotten via magnetic nanoparticles and dechlorination of trichloroethene coupled well with growth of Methanosarcina.

中文翻译：

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磁纳米粒子介导的混合脱氯混合培养促进全氯乙烯脱氯

高度富集的活性脱氯培养物在推进微生物修复技术中很重要。这项研究试图通过磁性纳米粒子介导的分离（MMI）来丰富快速的全氯乙烯（PCE）脱氯培养物。MMI是一种新颖的方法，可以在不进行标记的情况下分离微生物群落中快速增长和缓慢增长的种群。在MMI过程中，PCE的脱氯作用增强，但随后的三氯乙烯（TCE）脱氯作用受到抑制，TCE的累积率在70天内达到80.6％。同时，微生物群落也发生了变化，快速增长的属（如Dehalobacterium和Petrimonas）丰富，而缓慢生长的甲烷菌（Methanosarcina）几乎排除了。几个主要属（包括Petrimonas和Methanosarcina）的相对丰度与TCE的脱氯速率和Dehalococcoides的相对丰度呈正相关。在另一方面，Dehalobacterium呈负TCE脱氯率及相关Dehalococcoides丰富，这之间的潜在竞争Dehalobacterium和Dehalococcoides。甲烷菌的再生加上三氯乙烯（TCE）脱氯能力的恢复，这暗示了产甲烷菌在三氯乙烯（TCE）脱氯中的重要作用。通过MMI方法，可以建立一个更简单但活性更高的微生物联盟，以提高PCE修复效率。产甲烷菌可作为TCE脱氯的指示剂或生物标记，表明在富集脱氯培养物和修复受PCE污染的场所时，也应监测产甲烷的活性。

胶囊

通过磁性纳米粒子制备了一种快速的全氯乙烯脱氯剂，三氯乙烯的脱氯与甲烷藻的生长良好。