This article describes an original sol-gel (template) method for the synthesis of a dispersed form of nanostructured wollastonite (CaSiO₃), its gold nanoparticles (40–60 nm) functionalized form CaSiO₃/Au-NPs, and its composite in the composition with 20 mass% of hydroxyapatite CaSiO₃/HAp and synthetic hydroxyapatite, that are widely used in medicine for the reconstruction and regeneration of bone defects. The method provides the formation of porous silica carcass in samples with an average pore size of 100–160 nm due to the application of polymer siloxane-acrylate as a pore-forming template. The authors studied the processes of template destruction and phase formation in the composition of samples using TGA/DTA/DSC (thermogravimetric analysis/differential thermal analysis/differential scanning calorimetry), and XRD (X-ray diffraction analysis). The methods of SEM (scanning electron microscopy), low-temperature nitrogen adsorption, and mercury porosimetry were used to identify the morphology and study the structural characteristics (S_spec and V_por, pore size distribution) for dispersed nanostructured samples based on wollastonite and hydroxyapatite. An “in vitro” model was used to study the functional activity (metabolism and cytokine production) of innate immune cells (neutrophils and macrophages) in contact with the obtained samples and to evaluate the viability of cells, the activity of ATPase activity, the number of cationic proteins, and apoptosis of cells. The study is novel for these systems and contributes to the existing knowledge on the biocompatibility for “in vivo” model, which provides a complex evaluation of the quality of wollastonite biomaterials used as grafts.

本文介绍了一种原始的溶胶-凝胶（模板）方法，用于合成分散形式的纳米结构硅灰石 (CaSiO ₃ )、其金纳米粒子 (40–60 nm) 功能化形式的 CaSiO ₃ /Au-NPs 及其在含有20质量％羟基磷灰石CaSiO _{3 的}组合物/HAp 和合成羟基磷灰石，在医学上广泛用于骨缺损的重建和再生。由于使用聚合物硅氧烷-丙烯酸酯作为成孔模板，该方法可在平均孔径为 100-160 nm 的样品中形成多孔二氧化硅骨架。作者使用 TGA/DTA/DSC（热重分析/差热分析/差示扫描量热法）和 XRD（X 射线衍射分析）研究了样品组成中模板破坏和相形成的过程。采用SEM（扫描电子显微镜）、低温氮气吸附、压汞法等方法对其形貌进行鉴定并研究其结构特征（S _spec和V _por，孔径分布）用于基于硅灰石和羟基磷灰石的分散纳米结构样品。的“体外”模型被用于研究先天免疫细胞（嗜中性粒细胞和巨噬细胞）的功能活性（代谢和细胞因子产生）与所获得的样品接触，并评估细胞的生存力，ATP酶活性的活性，所述数阳离子蛋白和细胞凋亡。该研究对这些系统来说是新颖的，并有助于对“体内”模型生物相容性的现有知识做出贡献，该模型提供了对用作移植物的硅灰石生物材料质量的复杂评估。