Exposure to ultraviolet (UV) irradiation leads to the generation of reactive oxygen species (ROS) and DNA damage in skin tissue, which can further result in skin cancers. Using sunscreens is one of the most popular and the most effective method to resist UV irradiation. Resveratrol (RES) shows high absorbance in UV region and significant anti-oxidant effects. However, RES is easily degraded by UV irradiation, resulting in the decrease of bioactivity and the limitation of its application in the pharmaceutical preparations of skin. In this paper, a nanostructured lipid carrier gel loaded with RES (RES-NLC-gel) was prepared to improve the stability of RES and the accumulation of RES in the epidermis. Moreover, RES-NLC-gel could scavenge free radical effectively and protect human keratinocyte from UV irradiation by inhibiting the generation of ROS, decreasing the protein expression of cleaved caspase-3 and Bax and increasing the protein expression of Bcl-2. When mice skin was pretreated with RES-NLC-gel, there were less erythema, wrinkles and scabs on mice skin. The epidermal thickness of mice skins obviously reduced in dose-dependent manner. The activities of catalase (CAT), glutathione peroxidase (GPx) and superoxide dismutase (SOD) in mice skin tissue significantly increased. Thus, RES-NLC-gel exhibited an obvious anti-UV irradiation and anti-oxidant activity in vivo. RES-NLC-gel displayed great application potential in protecting skin from UV irradiation.

暴露于紫外线（UV）会导致皮肤组织中活性氧（ROS）的产生和DNA损伤，这可能进一步导致皮肤癌。使用防晒霜是抵抗紫外线辐射的最流行，最有效的方法之一。白藜芦醇（RES）在紫外线区具有高吸收率，并具有明显的抗氧化作用。然而，RES容易被紫外线辐射降解，导致生物活性降低，并且限制了其在皮肤药物制剂中的应用。本文制备了负载RES的纳米结构脂质载体凝胶（RES-NLC-gel），以提高RES的稳定性和RES在表皮中的积累。此外，RES-NLC凝胶可通过抑制ROS的产生来有效清除自由基并保护人类角质形成细胞免受紫外线辐射，降低了裂解的caspase-3和Bax的蛋白表达，并增加了Bcl-2的蛋白表达。当用RES-NLC-凝胶预处理小鼠皮肤时，小鼠皮肤上的红斑，皱纹和结sc较少。小鼠皮肤的表皮厚度以剂量依赖性方式明显降低。小鼠皮肤组织中的过氧化氢酶（CAT），谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）的活性显着增加。因此，RES-NLC凝胶表现出明显的抗紫外线辐射和抗氧化活性 小鼠皮肤组织中的谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）明显增加。因此，RES-NLC凝胶表现出明显的抗紫外线辐射和抗氧化活性 小鼠皮肤组织中的谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）明显增加。因此，RES-NLC凝胶表现出明显的抗紫外线辐射和抗氧化活性体内。RES-NLC凝胶在保护皮肤免受紫外线辐射方面显示出巨大的应用潜力。