用于活细胞中次氯酸实时检测和成像的灵敏且选择性的荧光探针,ACS Omega

当前位置： X-MOL 学术 › ACS Omega › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

用于活细胞中次氯酸实时检测和成像的灵敏且选择性的荧光探针
ACS Omega ( IF 3.7 ) Pub Date : 2021-04-26 , DOI: 10.1021/acsomega.1c01102
Pan Luo ₁ , Xuejun Zhao ₂

Affiliation

次氯酸（HClO）是一种活性氧，通过与大多数生物分子发生反应，在生理学和病理学过程中起着至关重要的作用。HClO含量异常可能会引起炎症，尤其是关节炎。为了进一步了解其在炎症中的关键作用，必须对HClO进行原位检测。本文中，水溶性小分子荧光探针（HDI-HClO）用于监测和鉴定生物系统中的痕量HClO。在HClO存在下，由于分子内电荷转移过程的恢复，探针释放出释放强荧光的羟基。此外，该探针显示出150倍的荧光增强，并伴有较大的斯托克斯位移和较低的检测限（8.3 nM）。此外，该探针可在8 s内对HClO做出快速响应，这提供了实时监测细胞内HClO的可能性。基于快速动力学，良好的水溶性和出色的生物相容性的优点，该探针可有效监测活细胞中外源和内源HClO的波动。HDI-HClO的荧光成像表明，它是理解炎症与HClO之间关系的极好的潜在方法。

"点击查看英文标题和摘要"

A Sensitive and Selective Fluorescent Probe for Real-Time Detection and Imaging of Hypochlorous Acid in Living Cells

Hypochlorous acid (HClO), a reactive oxygen species, plays an essential role in the processes of physiology and pathology via reacting with most biological molecules. The abnormal level of HClO may cause inflammation, especially arthritis. To further understand its key role in inflammation, in situ detection of HClO is necessary. Herein, a water-soluble small molecule fluorescent probe (HDI-HClO) is employed to monitor and identify trace amounts of HClO in the biological system. In the presence of HClO, the probe releases a hydroxyl group emitting strong fluorescence because of the restoration of the intramolecular charge transfer process. Furthermore, this probe displays a 150-fold fluorescence enhancement accompanied by a large Stokes shift and a lower detection limit (8.3 nM). Moreover, the probe can make a rapid response to HClO within 8 s, which provides the possibility of real-time monitoring of intracellular HClO. Based on the advantages of rapid dynamics, good water solubility, and excellent biocompatibility, this probe could effectively monitor the fluctuations of exogenous and endogenous HClO in living cells. The fluorescence imaging of HDI-HClO indicated that it is an excellent potential approach for comprehending the relationship between inflammation and HClO.

更新日期：2021-05-11

点击分享查看原文

点击收藏

公开下载

阅读更多本刊新发论文本刊介绍/投稿指南