The Glycyl Radical Enzyme Arylacetate Decarboxylase from Olsenella scatoligenes,ACS Catalysis

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The Glycyl Radical Enzyme Arylacetate Decarboxylase from Olsenella scatoligenes
ACS Catalysis ( IF 11.3 ) Pub Date : 2021-04-27 , DOI: 10.1021/acscatal.1c01253
Qiang Lu _{1,

2} , Yifeng Wei ₃ , Lianyun Lin ₁ , Jiayi Liu ₁ , Yongxu Duan ₁ , Yaxin Li ₁ , Weixiang Zhai ₄ , Yangping Liu ₄ , Ee Lui Ang ₃ , Huimin Zhao _{3,

5} , Zhiguang Yuchi ₁ , Yan Zhang _{1,

2}

Affiliation

The glycyl radical enzymes (GREs) p-hydroxyphenylacetate (HPA) decarboxylase (HPAD), indoleacetate decarboxylase, and phenylacetate decarboxylase catalyze the radical-mediated decarboxylation of arylacetates, which are products of bacterial aromatic amino acid fermentation. Here, we report the discovery and structural and biochemical investigation of a fourth GRE arylacetate decarboxylase (AAD) from Olsenella scatoligenes that catalyzes HPA decarboxylation. AAD also catalyzes the decarboxylation of p-aminophenylacetate, which is not a substrate of HPAD, and lacks the Fe–S cluster containing small subunit. The structure of AAD in complex with p-hydroxyphenylacetate was determined by X-ray crystallography. The differing substrate ranges and active site structures of AAD and HPAD suggest distinct catalytic mechanisms, underscoring the diversity of radical-mediated decarboxylation reactions.

中文翻译：

糖化Olsenella scatoligenes的糖基自由基酶芳基乙酸酯脱羧酶

糖基自由基酶（GREs）的对羟基苯乙酸酯（HPA）脱羧酶（HPAD），吲哚乙酸酯脱羧酶和苯乙酸酯脱羧酶催化自由基介导的芳基乙酸酯的脱羧反应，这是细菌芳香族氨基酸发酵的产物。在这里，我们报告发现和结构和生化研究的第四个GRE芳基乙酸酯脱羧酶（AAD）从产油青霉的催化HPA脱羧。AAD还催化不是HPAD底物的对-氨基苯乙酸酯的脱羧作用，并且缺少含有小的亚基的Fe–S簇。与p复数的AAD的结构通过X-射线晶体学测定乙酸-羟基苯酯。AAD和HPAD的不同底物范围和活性位点结构表明不同的催化机制，从而强调了自由基介导的脱羧反应的多样性。

更新日期：2021-05-07

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