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Hypermineralization of Hearing-Related Bones by a Specific Osteoblast Subtype
Journal of Bone and Mineral Research ( IF 5.1 ) Pub Date : 2021-04-27 , DOI: 10.1002/jbmr.4320
Yukiko Kuroda 1 , Katsuhiro Kawaai 1 , Naoya Hatano 2 , Yanlin Wu 3 , Hidekazu Takano 3 , Atsushi Momose 3 , Takuya Ishimoto 4 , Takayoshi Nakano 4 , Paul Roschger 5 , Stéphane Blouin 5 , Koichi Matsuo 1
Affiliation  

Auditory ossicles in the middle ear and bony labyrinth of the inner ear are highly mineralized in adult mammals. Cellular mechanisms underlying formation of dense bone during development are unknown. Here, we found that osteoblast-like cells synthesizing highly mineralized hearing-related bones produce both type I and type II collagens as the bone matrix, while conventional osteoblasts and chondrocytes primarily produce type I and type II collagens, respectively. Furthermore, these osteoblast-like cells were not labeled in a “conventional osteoblast”-specific green fluorescent protein (GFP) mouse line. Type II collagen-producing osteoblast-like cells were not chondrocytes as they express osteocalcin, localize along alizarin-labeled osteoid, and form osteocyte lacunae and canaliculi, as do conventional osteoblasts. Auditory ossicles and the bony labyrinth exhibit not only higher bone matrix mineralization but also a higher degree of apatite orientation than do long bones. Therefore, we conclude that these type II collagen-producing hypermineralizing osteoblasts (termed here auditory osteoblasts) represent a new osteoblast subtype. © 2021 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR).

中文翻译:

特定成骨细胞亚型对听力相关骨骼的过度矿化

中耳的听小骨和内耳的骨迷路在成年哺乳动物中高度矿化。发育过程中致密骨形成的细胞机制尚不清楚。在这里,我们发现合成高度矿化的听力相关骨骼的成骨细胞样细胞产生 I 型和 II 型胶原蛋白作为骨基质,而传统的成骨细胞和软骨细胞分别主要产生 I 型和 II 型胶原蛋白。此外,这些成骨细胞样细胞未在“常规成骨细胞”特异性绿色荧光蛋白 (GFP) 小鼠系中标记。产生 II 型胶原蛋白的成骨细胞样细胞不是软骨细胞,因为它们表达骨钙素,沿着茜素标记的类骨质定位,并形成骨细胞腔隙和小管,就像传统的成骨细胞一样。与长骨相比,听小骨和骨迷路不仅表现出更高的骨基质矿化,而且还表现出更高程度的磷灰石取向。因此,我们得出结论,这些产生 II 型胶原蛋白的超矿化成骨细胞(此处称为听觉成骨细胞)代表了一种新的成骨细胞亚型。© 2021 作者。《骨骼与矿物研究杂志》由 Wiley Periodicals LLC 代表美国骨骼与矿物研究学会 (ASBMR) 出版。
更新日期:2021-04-27
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