Affinity chromatography is a standard method, which used for protein purification and separation studies due to its specificity and selectivity. There are several affinity chromatography methods, such as dye affinity, immobilized metal chelated affinity, and affinity electrophoresis. Cibacron Blue F3G-A (CBD), as a dye ligand, is one of the most used dyes among dye affinity chromatography. CBD is ideally suited for human serum albumin (HSA) separation and purification in affinity chromatography for several years. However, even though CBD has many purification applications, there is not much research focused on the interaction between CBD and HSA in molecular docking. The interactions between CBD and HSA were simulated via AutoDock molecular docking software in this study. Investigated possibilities resulted in six different conformations on different locations, which light the way to variable connectivity of CBD. Thus, it was determined that the most favorable binding is conformation 5, with its lowest binding energy, which is energetically favorable.

亲和色谱法是一种标准方法，由于其特异性和选择性，可用于蛋白质纯化和分离研究。有几种亲和色谱方法，例如染料亲和，固定化金属螯合亲和和亲和电泳。Cibacron Blue F3G-A（CBD）作为染料配体，是染料亲和色谱法中最常用的染料之一。CBD非常适合在亲和色谱中分离和纯化人血清白蛋白（HSA）数年。但是，即使CBD具有许多纯化应用，也没有太多的研究集中在CBD和HSA在分子对接中的相互作用。在这项研究中，通过AutoDock分子对接软件模拟了CBD和HSA之间的相互作用。研究的可能性导致在不同位置出现了六个不同的构象，这为CBD的可变连通性开辟了道路。因此，确定最有利的结合是构型5，其结合能最低，这在能量上是有利的。