Abstract

The high incidence of cardiovascular disease has led to significant demand for synthetic vascular grafts in clinical applications. Anti-proliferation drugs are usually loaded into devices to achieve desirable anti-thrombosis effects after implantation. However, the non-selectiveness of these conventional drugs can lead to the failure of blood vessel reconstruction, leading to potential complications in the long term. To address this issue, an asymmetric membrane was constructed through electro-spinning techniques. The bilayer membrane loaded and effectively released nitric oxide (NO), as hoped, from only one side. Due to the short diffusion distance of NO, it exerted negligible effects on the other side of the membrane, thus allowing selective regulation of different cells on both sides. The released NO boosted the growth of endothelial cells (ECs) over smooth muscle cells (SMCs)—while on the side where NO was absent, SMCs grew into multilayers. The overall structure resembled a native blood vessel, with confluent ECs as the inner layer and layers of SMCs to support it. In addition, the membrane preserved the normal function of ECs, and at the same time did not exacerbate inflammatory responses. By preparing this material type that regulates cell behavior differentially, we describe a new method for its application in the cardiovascular field such as for artificial blood vessels.

Graphic abstract

中文翻译：

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具有不对称NO释放功能的电纺纤维膜，用于细胞生长的差异调节

摘要

心血管疾病的高发病率导致在临床应用中对合成血管移植物的大量需求。通常将抗增殖药物装载到装置中，以在植入后达到理想的抗血栓形成作用。然而，这些常规药物的非选择性可导致血管重建失败，长期而言可能导致潜在的并发症。为了解决这个问题，通过电纺丝技术构造了不对称膜。如所希望的那样，双层膜仅从一侧装载并有效释放了一氧化氮（NO）。由于NO的扩散距离短，它在膜的另一侧可忽略不计，因此可以选择性调节两侧的不同细胞。释放的NO促进了内皮细胞（EC）的生长，超过了平滑肌细胞（SMC）的生长-而在没有NO的一侧，SMC则生长为多层。总体结构类似于天然血管，融合的EC作为内层，SMC层支撑着它。另外，该膜保留了EC的正常功能，并且同时没有加剧炎症反应。通过准备这种可以差异调节细胞行为的材料类型，我们描述了一种在心血管领域（例如人造血管）中应用的新方法。膜保留了EC的正常功能，但同时没有加剧炎症反应。通过准备这种可以差异调节细胞行为的材料类型，我们描述了一种在心血管领域（例如人造血管）中应用的新方法。膜保留了EC的正常功能，但同时没有加剧炎症反应。通过准备这种可以差异调节细胞行为的材料类型，我们描述了一种在心血管领域（例如人造血管）中应用的新方法。

图形摘要