Solvents exhibit different color reactions via regulation of the structure, kinetics, and charge distribution of organic compound molecules. In recent years, solvatochromic probes have good application prospects in the fields of chemistry and biology. In this work, we synthesized three fluorescent probes (PBIC–C, PAIC-C, PPIC-C) sensitive to solvent polarity. We have studied the photophysical properties (spectra, quantum yield, and fluorescence lifetime under different solvents) of these probes. The isomerization nature was verified by nuclear magnetic titration. In order to further confirm our guess, we also performed theoretical calculations, and the results were roughly the same as the experimental results. In addition, cell imaging tests revealed that the designed probes could be used as dual intracellular mitochondria/lysosome-targeting fluorescent probes for biological applications.

溶剂通过调节有机化合物分子的结构，动力学和电荷分布表现出不同的颜色反应。近年来，溶剂变色探针在化学和生物学领域具有良好的应用前景。在这项工作中，我们合成了三种对溶剂极性敏感的荧光探针（PBIC-C，PAIC-C，PPIC-C）。我们已经研究了这些探针的光物理性质（光谱，量子产率和在不同溶剂下的荧光寿命）。通过核磁滴定法证实了异构化性质。为了进一步证实我们的猜测，我们还进行了理论计算，结果与实验结果大致相同。此外，