Realization of noncontact confocal optical microsphere imaging microscope,Microscopy Research and Technique

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Realization of noncontact confocal optical microsphere imaging microscope
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2021-04-21 , DOI: 10.1002/jemt.23793
Yan Zhou ₁ , Minghui Hong ₁

Affiliation

A novel noncontact confocal optical microsphere imaging microscope (COMIM) is proposed to achieve high contrast optical nano-imaging at a high scanning speed. The developed setup obtains images with significantly higher contrast when compared with conventional bright-field microsphere nano-imaging, as well as with a commercial confocal laser scanning microscope (CLSM). With the image-by-image scanning scheme, COMIM takes only 11% time to complete a three-dimensional (3D) scanning image than using a commercial CLSM, while still offers an outstanding resolving power for the nano-features on the sample.

中文翻译：

非接触式共焦光学微球成像显微镜的实现

提出了一种新型非接触式共焦光学微球成像显微镜 (COMIM)，以实现高扫描速度下的高对比度光学纳米成像。与传统的明场微球纳米成像以及商业共聚焦激光扫描显微镜 (CLSM) 相比，开发的设置获得的图像对比度明显更高。通过逐个图像扫描方案，COMIM 完成三维 (3D) 扫描图像的时间比使用商用 CLSM 只需要 11% 的时间，同时仍然为样品上的纳米特征提供出色的分辨率。

更新日期：2021-04-21

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