血清对具有银纳米颗粒标记的生物测定的电化学检测的影响,ACS Sensors

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血清对具有银纳米颗粒标记的生物测定的电化学检测的影响
ACS Sensors ( IF 8.2 ) Pub Date : 2021-04-22 , DOI: 10.1021/acssensors.1c00446
Nicole E Pollok ₁ , Yi Peng ₁ , Charlie Rabin ₁ , Ian Richards ₂ , Richard M Crooks ₁

Affiliation

研究了血清对具有银纳米颗粒（AgNP）检测标记的生物测定的电化学检测的影响。对心力衰竭标记物 NT-proBNP 的模型测定和抗原特异性夹心生物测定进行了检查。在这两种情况下，AgNP 标记均与检测抗体结合。使用电流交换/阳极溶出伏安法进行电化学检测，其中Au ³⁺与AgNP 标记进行交换。使用纸基电极平台进行测定。在检测之前和检测期间将生物测定暴露于不同的血清条件。本文报告了三个重要成果。首先，模型特异性和抗原特异性检测都可以在未稀释的血清中形成，没有可检测到的血清成分干扰。其次，为了获得最大可能的电化学信号，在电化学检测过程中，在不进行洗涤的情况下，测定缓冲液中可以保留的最高血清百分比为 0.25%。当存在 0.50% 或更多血清时，测定结果将变得不准确。第三，抑制血清中电交换的因素可能与AgNP标记上生物分子的表面吸附、血清成分对Au ³⁺的螯合或两者有关。这里报告的结果为使用金属纳米粒子标记进行生物流体电化学测定提供了一般指导。

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Effect of Serum on Electrochemical Detection of Bioassays Having Ag Nanoparticle Labels

The effect of serum on electrochemical detection of bioassays having silver nanoparticle (AgNP) detection labels was investigated. Both a model assay and an antigen-specific sandwich bioassay for the heart failure marker NT-proBNP were examined. In both cases, the AgNP labels were conjugated to a detection antibody. Electrochemical detection was carried out using a galvanic exchange/anodic stripping voltammetry method in which Au³⁺ exchanges with AgNP labels. The assays were carried out using a paper-based electrode platform. The bioassays were exposed to different serum conditions prior to and during detection. There are three important outcomes reported in this article. First, both the model- and antigen-specific assays could be formed in undiluted serum with no detectable interferences from the serum components. Second, to achieve the maximum possible electrochemical signal, the highest percentage of serum that can remain in an assay buffer during electrochemical detection is 0.25% when no washing is performed. The assay results are rendered inaccurate when 0.50% or more of serum is present. Third, the factors inhibiting galvanic exchange in serum probably relate to surface adsorption of biomolecules onto the AgNP labels, chelation of Au³⁺ by serum components, or both. The results reported here provide general guidance for using metal NP labels for electrochemical assays in biofluids.

更新日期：2021-05-28

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