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Sensitive Dual-Mode Biosensors for CYFRA21-1 Assay Based on the Dual-Signaling Electrochemical Ratiometric Strategy and “On–Off–On” PEC Method
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-04-20 , DOI: 10.1021/acs.analchem.1c00746
Qingqing Zhang 1 , Yamin Fu 1 , Ke Xiao 1 , Cuicui Du 1 , Xiaohua Zhang 1 , Jinhua Chen 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2021-04-20 , DOI: 10.1021/acs.analchem.1c00746
Qingqing Zhang 1 , Yamin Fu 1 , Ke Xiao 1 , Cuicui Du 1 , Xiaohua Zhang 1 , Jinhua Chen 1
Affiliation
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Herein, an electrochemical (EC)–photoelectrochemical (PEC) dual-mode biosensor was constructed for cytokeratin 19 fragment 21-1 (CYFRA21-1) assay based on the dual-signaling electrochemical ratiometric strategy and “on–off–on” PEC method. The indium tin oxide (ITO) electrode was modified by 3,4,9,10-perylenetetracarboxylic dianhydride (PTCDA)@C60 and gold nanoparticles (Au NPs), and the double-stranded DNA composed of thiol/methylene blue (MB)-labeled single-stranded DNA (ssDNA) (S0-MB) and antibody/ferrocene (Fc)-labeled ssDNA (Ab1-S1-Fc) was immobilized on the Au NPs/PTCDA@C60/ITO electrode via the Au–S bond between Au NPs and thiol of S0-MB. With the help of another antibody-labeled ssDNA (Ab2-S2), the presence of CYFRA21-1 triggered a typical antigen–antibody sandwich immune reaction (Ab1, CYFRA21-1, and Ab2) and proximity hybridization between Ab1-S1-Fc and Ab2-S2. This caused the release of Ab1-S1-Fc from the modified electrode and the change of S0-MB to a hairpin structure, resulting in a decrease (an increase) of the oxidation peak current of Fc (MB) and an increase of the photocurrent due to the enhancing (inhibiting) effect of MB (Fc) on the photoelectric performance of the Au NPs/PTCDA@C60/ITO electrode. Thus, CYFRA21-1 was detected by the developed EC–PEC dual-mode sensing platform sensitively, and the linear response ranges of 0.001–40 ng/mL with a detection limit of 0.3 pg/mL for the EC technique and 0.0001–4 ng/mL with a detection limit of 0.03 pg/mL for the PEC method were obtained. Furthermore, by changing the specific antibodies of disease-related biomarkers, the developed dual-mode biosensing platform could be readily extended to detect other antigens, implying its great potential applications in biological analysis and early disease diagnosis.
中文翻译:
基于双信号电化学比例策略和“开-关-开” PEC方法的用于CYFRA21-1分析的灵敏双模式生物传感器
在此,基于双信号电化学比例法和“开-关-开” PEC方法,构建了用于细胞角蛋白19片段21-1(CYFRA21-1)测定的电化学(EC)-光电化学(PEC)双模式生物传感器。 。氧化铟锡(ITO)电极用3,4,9,10-per四甲酸二酐(PTCDA)@C 60和金纳米颗粒(Au NPs)修饰,并且由硫醇/亚甲基蓝(MB)组成的双链DNA标记的单链DNA(ssDNA)(S0-MB)和抗体/二茂铁(Fc)标记的ssDNA(Ab1-S1-Fc)被固定在Au NPs / PTCDA @ C 60上/ ITO电极通过Au NP和S0-MB的硫醇之间的Au-S键。借助于另一种抗体标记的ssDNA(Ab2-S2),CYFRA21-1的存在触发了典型的抗原-抗体夹心免疫反应(Ab1,CYFRA21-1和Ab2),并在Ab1-S1-Fc和Ab2-S2。这引起了Ab1-S1-Fc从修饰电极的释放和S0-MB到发夹结构的变化,导致Fc(MB)氧化峰电流的减少(增加)和光电流的增加由于MB(Fc)对Au NPs / PTCDA @ C 60的光电性能的增强(抑制)作用/ ITO电极。因此,已开发的EC–PEC双模式传感平台可灵敏地检测到CYFRA21-1,其线性响应范围为0.001–40 ng / mL,EC技术的检出限为0.3 pg / mL,0.0001–4 ng / PEC方法的检出限为0.03 pg / mL。此外,通过改变疾病相关生物标志物的特异性抗体,可以轻松扩展已开发的双模式生物传感平台,以检测其他抗原,这意味着其在生物学分析和疾病早期诊断中的巨大潜力。
更新日期:2021-05-04
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
基于双信号电化学比例策略和“开-关-开” PEC方法的用于CYFRA21-1分析的灵敏双模式生物传感器
在此,基于双信号电化学比例法和“开-关-开” PEC方法,构建了用于细胞角蛋白19片段21-1(CYFRA21-1)测定的电化学(EC)-光电化学(PEC)双模式生物传感器。 。氧化铟锡(ITO)电极用3,4,9,10-per四甲酸二酐(PTCDA)@C 60和金纳米颗粒(Au NPs)修饰,并且由硫醇/亚甲基蓝(MB)组成的双链DNA标记的单链DNA(ssDNA)(S0-MB)和抗体/二茂铁(Fc)标记的ssDNA(Ab1-S1-Fc)被固定在Au NPs / PTCDA @ C 60上/ ITO电极通过Au NP和S0-MB的硫醇之间的Au-S键。借助于另一种抗体标记的ssDNA(Ab2-S2),CYFRA21-1的存在触发了典型的抗原-抗体夹心免疫反应(Ab1,CYFRA21-1和Ab2),并在Ab1-S1-Fc和Ab2-S2。这引起了Ab1-S1-Fc从修饰电极的释放和S0-MB到发夹结构的变化,导致Fc(MB)氧化峰电流的减少(增加)和光电流的增加由于MB(Fc)对Au NPs / PTCDA @ C 60的光电性能的增强(抑制)作用/ ITO电极。因此,已开发的EC–PEC双模式传感平台可灵敏地检测到CYFRA21-1,其线性响应范围为0.001–40 ng / mL,EC技术的检出限为0.3 pg / mL,0.0001–4 ng / PEC方法的检出限为0.03 pg / mL。此外,通过改变疾病相关生物标志物的特异性抗体,可以轻松扩展已开发的双模式生物传感平台,以检测其他抗原,这意味着其在生物学分析和疾病早期诊断中的巨大潜力。