The interaction between proteins and drugs/ligands has been explored thoroughly over the past years. Numerous spectroscopic and in silico tools are employed to decipher the binding mechanism of drugs/ligands with serum albumin. Serum albumin is the principal component present in the blood plasma and is responsible for carrying both exogenous and endogenous substances to its target site. The binding affinity between drugs/ligands and serum albumin plays a significant role in designing novel drugs/ligands and altering the doses of pre-existing drugs/ligands to achieve the desired therapeutic efficacy. This review mainly focuses on various techniques used to study the binding mechanism involved between drugs/ligands and serum albumin. Moreover, several techniques that can be implemented to explore the conformational changes induced in serum albumin during the transport process are also discussed. These techniques come up with data that are simple to interpret and give reliable information regarding the interaction between therapeutic molecules and proteins.

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研究药物/配体与血清白蛋白的相互作用


在过去的几年里，蛋白质与药物/配体之间的相互作用已经得到了彻底的探索。许多光谱和计算机工具被用来破译药物/配体与血清白蛋白的结合机制。血清白蛋白是血浆中存在的主要成分，负责将外源性和内源性物质携带到其目标部位。药物/配体与血清白蛋白之间的结合亲和力在设计新型药物/配体和改变预先存在的药物/配体的剂量以实现所需的治疗效果中起着重要作用。本文主要综述用于研究药物/配体与血清白蛋白之间结合机制的各种技术。此外，还讨论了几种可用于探索血清白蛋白在运输过程中诱导的构象变化的技术。这些技术得出的数据易于解释，并提供有关治疗分子和蛋白质之间相互作用的可靠信息。