Hyaluronic acid (HA) solutions were crosslinked with divinyl sulfone (DVS) and subsequently loaded with antibiotic molecules to obtain biocompatible and antibacterial injectable hydrogels. The crosslinking degree of the hydrogels was modulated by varying the reaction time and the HA:DVS weight ratio. Synthesized HA-DVS hydrogels were characterized by their rheological properties, pore size, swelling capacity and hydrolytic and thermal degradation. Biocompatibility was assessed by measuring pH, osmolality and by in vitro cytotoxic assay. Acetyl salicylic (AAS) loaded hydrogels display anti-inflammatory properties in vitro, whereas cefuroxime (CFX), tetracycline (TCN) and amoxicillin (AMX) loaded hydrogels show in vitro antibacterial activity against Staphylococcus aureus. The combine use of antibiotics and AAS produces a synergic effect that reduces the S. aureus population up to a log10 reduction (R) of 5.55. Overall results show that antibiotic/AAS loaded HA-DVS hydrogels could be effectively used to combat S. aureus infections and to increase the antibacterial activity of antibiotics commonly used against S. aureus.

将透明质酸（HA）溶液与二乙烯基砜（DVS）交联，然后加载抗生素分子以获得生物相容性和抗菌性可注射水凝胶。通过改变反应时间和HA∶DVS重量比来调节水凝胶的交联度。合成的HA-DVS水凝胶的特征是其流变性，孔径，溶胀能力以及水解和热降解。通过测量pH，重量克分子渗透压浓度和体外细胞毒性测定法评估生物相容性。乙酰水杨酸（AAS）负载的水凝胶在体外显示抗炎特性，而头孢呋辛（CFX），四环素（TCN）和阿莫西林（AMX）负载的水凝胶在体外显示出对细菌的抗菌活性。金黄色葡萄球菌。抗生素和AAS的联合使用产生协同作用，可将金黄色葡萄球菌种群减少至log10降低（R）为5.55。总体结果表明，载有抗生素/ AAS的HA-DVS水凝胶可有效地对抗金黄色葡萄球菌感染，并提高常用的针对金黄色葡萄球菌的抗生素的抗菌活性。