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The Most Active Oxidase-Mimicking Mn2O3 Nanozyme for Biosensor Signal Generation
Chemistry - A European Journal ( IF 3.9 ) Pub Date : 2021-04-15 , DOI: 10.1002/chem.202100567
Zi-Jian Chen 1, 2 , Zhicheng Huang 2 , Yuan-Ming Sun 1 , Zhen-Lin Xu 1 , Juewen Liu 2
Chemistry - A European Journal ( IF 3.9 ) Pub Date : 2021-04-15 , DOI: 10.1002/chem.202100567
Zi-Jian Chen 1, 2 , Zhicheng Huang 2 , Yuan-Ming Sun 1 , Zhen-Lin Xu 1 , Juewen Liu 2
Affiliation
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Oxidase-mimicking nanozymes are more desirable than peroxidase-mimicking ones since H2O2 can be omitted. However, only a few nanomaterials are known for oxidase-like activities. In this work, we compared the activity of Mn2O3, Mn3O4 and MnO2 and found that Mn2O3 had the highest oxidase activity. Interestingly, the activity of Mn2O3 was even inhibited by H2O2. The oxidase-like activity of Mn2O3 was not much affected by the presence of proteins such as bovine serum albumin (BSA), but the physisorption of antibodies to Mn2O3 was not strong enough to withstand the displacement by BSA. We then treated Mn2O3 with 3-aminopropyltriethoxysilane to graft an amine group, which was used to conjugate antibodies using glutaraldehyde as a crosslinker. A one-step indirect competitive ELISA (icELISA) was developed for the detection of isocarbophos, and an IC50 of 261.7 ng/mL was obtained, comparable with the results of the standard two-step assay using horseradish peroxidase (HRP)-labeled antibodies. This assay has the advantage of significant timesaving for rapid detection of large amounts of samples. This work has discovered a highly efficient oxidase-mimicking nanozyme useful for various nano- and analytical applications.
中文翻译：
用于生物传感器信号生成的最活跃的氧化酶模拟 Mn2O3 纳米酶
氧化酶模拟纳米酶比过氧化物酶模拟纳米酶更受欢迎，因为可以省略H 2 O 2。然而，只有少数纳米材料具有类似氧化酶的活性。在这项工作中，我们比较了Mn 2 O 3、Mn 3 O 4和MnO 2的活性，发现Mn 2 O 3具有最高的氧化酶活性。有趣的是，Mn 2 O 3的活性甚至被H 2 O 2抑制。Mn 2 O 3的氧化酶样活性受牛血清白蛋白 (BSA) 等蛋白质存在的影响不大，但 Mn 2 O 3抗体的物理吸附强度不足以承受 BSA 的置换。然后我们用 3-氨基丙基三乙氧基硅烷处理 Mn 2 O 3以接枝胺基团，该基团用于使用戊二醛作为交联剂偶联抗体。开发了一种用于检测异卡波磷的一步间接竞争 ELISA (icELISA)，IC 50获得了 261.7 ng/mL，与使用辣根过氧化物酶 (HRP) 标记抗体的标准两步法测定结果相当。该测定具有显着节省大量样品快速检测时间的优点。这项工作发现了一种高效的氧化酶模拟纳米酶，可用于各种纳米和分析应用。
更新日期：2021-04-15
中文翻译：
用于生物传感器信号生成的最活跃的氧化酶模拟 Mn2O3 纳米酶
氧化酶模拟纳米酶比过氧化物酶模拟纳米酶更受欢迎，因为可以省略H 2 O 2。然而，只有少数纳米材料具有类似氧化酶的活性。在这项工作中，我们比较了Mn 2 O 3、Mn 3 O 4和MnO 2的活性，发现Mn 2 O 3具有最高的氧化酶活性。有趣的是，Mn 2 O 3的活性甚至被H 2 O 2抑制。Mn 2 O 3的氧化酶样活性受牛血清白蛋白 (BSA) 等蛋白质存在的影响不大，但 Mn 2 O 3抗体的物理吸附强度不足以承受 BSA 的置换。然后我们用 3-氨基丙基三乙氧基硅烷处理 Mn 2 O 3以接枝胺基团，该基团用于使用戊二醛作为交联剂偶联抗体。开发了一种用于检测异卡波磷的一步间接竞争 ELISA (icELISA)，IC 50获得了 261.7 ng/mL，与使用辣根过氧化物酶 (HRP) 标记抗体的标准两步法测定结果相当。该测定具有显着节省大量样品快速检测时间的优点。这项工作发现了一种高效的氧化酶模拟纳米酶，可用于各种纳米和分析应用。
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