In this study, seven laccase genes from different bacteria were linked with the signal peptides PelB, Lpp or Ompa for heterologous expression in E. coli. The recombinant strains were applied for the removal of sulfadiazine (SDZ), sulfamethazine (SMZ), and sulfamethoxazole (SMX). The results obtained for different signal peptides did not provide insights into the removal mechanism. The removal ratios of SDZ, SMZ and SMX obtained with the recombinant strain 6#P at 60 h were around 92.0%, 89.0%, and 88.0%, respectively. The degradation pathways of sulfonamides have been proposed, including SO₂ elimination, hydroxylation, oxidation, pyrimidine ring cleavage, and N-S bond cleavage. Different mediators participate in the degradation of antibiotics through different mechanisms, and different antibiotics have different responses to the same mediator. The addition of 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) slightly promoted the removal of sulfonamides by most recombinant strains with different signal peptides, especially for the recombinant strain 2#O. The removal of sulfonamides by 1-hydroxybenzotriazole (HBT) varied with the recombinant strains. Syringaldehyde (SA) had a slight inhibitory effect on the removal of sulfonamides, with the most significant effect on strains 7#L and 7#O.

在这项研究中，来自不同细菌的七个漆酶基因与信号肽 PelB、Lpp 或 Ompa 相关联，以便在大肠杆菌中进行异源表达。重组菌株用于去除磺胺嘧啶 (SDZ)、磺胺二甲嘧啶 (SMZ) 和磺胺甲恶唑 (SMX)。对不同信号肽获得的结果没有提供对去除机制的深入了解。重组菌株6#P在60  h对SDZ、SMZ和SMX的去除率分别约为92.0%、89.0%和88.0%。已经提出了磺胺类的降解途径，包括 SO ₂消除、羟基化、氧化、嘧啶环裂解和 NS 键裂解。不同的介质通过不同的机制参与抗生素的降解，不同的抗生素对同一介质有不同的反应。2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) 的加入对大多数具有不同信号肽的重组菌株，特别是重组菌株2#O 对磺胺类药物的去除有轻微的促进作用。1-羟基苯并三唑 (HBT) 对磺胺的去除因重组菌株而异。丁香醛(SA)对磺胺类药物的去除有轻微的抑制作用，对7#L和7#O菌株的影响最为显着。